Analysis of F2-Isoprostanes by Gas Chromatography-Mass Spectrometry/Negative Ion Chemical Ionization

  • L. Jackson RobertsII
  • Jason D. Morrow
Part of the Methods in Pharmacology and Toxicology book series (MIPT)


Numerous methods have been developed to assess oxidative stress/injury in vivo in humans, which include products of oxidation of lipids, proteins, and DNA. However, it has long been recognized that most methods are unreliable because they measure products that are not specific products of free radical-induced oxidation, because the method is not specific for the oxidation product being measured, or because the method is too invasive for use in humans (1). Thus, it has long been recognized that there has been a need for a reliable method to assess oxidative stress status in vivo in humans.


Oxidative Stress Status Phosphomolybdic Acid Glass Centrifuge Tube Glass Scintillation Vial Pentafluorobenzyl Bromide 
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  1. 1.
    Halliwell, B. (1987) The measurement of free radical reactions in humans. FEBS Lett. 213, 9–14.PubMedCrossRefGoogle Scholar
  2. 2.
    Morrow, J. D., Hill, K. E., Burk, R. F., Nammour, T. M., Badr, K. F., and Roberts, L. J. II (1990) A series of prostaglandin F2-like compounds are produced in vivos in humans by a non-cyclooxygenase free radical catalyzed mechanism. Proc. Natl. Acad. Sci. USA 87, 9383–9387.PubMedCrossRefGoogle Scholar
  3. 3.
    Roberts, L. J. II and Morrow, J. D. (2000) Measurement of F2-isoprostanes as an index of oxidative stress in vivo. Free Rad. Biol. Med. 28, 505–513.PubMedCrossRefGoogle Scholar
  4. 4.
    Pryor, W. (2000) Oxidative stress status: the sec set. Free Rad. Biol. Med. 28, 503–504.PubMedCrossRefGoogle Scholar
  5. 5.
    Morrow, J. D., Awad, J. A., Boss, H. J., Blair, I. A., and Roberts, L. J. II (1992) Non-cyclooxygenase derived prostanoids (F2-isoprostanes) are formed in situ on phospholipids. Proc. Natl. Acad. Sci. USA 89, 10721–10725.PubMedCrossRefGoogle Scholar
  6. 6.
    Morrow, J. D., Minton, T. A., Mukundan, C. R., Campbell, M. D., Zackert, W. E., Daniel, V. C., et al. (1994) Free radical induced generation of isoprostanes in vivo: Evidence for the formation of D-ring and E-ring isoprostanes. J. Biol. Chem. 269, 4317–4326.PubMedGoogle Scholar
  7. 7.
    Morrow, J. D., Harris, T. M., and Roberts, L. J. II (1990) Non-cyclooxygenase oxidative formation of a series of novel prostaglandins: Analytical ramifications for measurement of eicosanoids. Anal. Biochem. 184, 1–10.PubMedCrossRefGoogle Scholar
  8. 8.
    Morrow, J. D., Badr, K. F., and Roberts, L. J. II (1994) Evidence that the F2-isoprostane, 8-epi-PGF2a, is formed in vivo. Biochim. Biophys. Acta. 1210, 244–248.PubMedGoogle Scholar
  9. 9.
    Morrow, J. D. and Roberts, L. J. II (1998) Mass spectrometric quantification of F2-isoprostanes in biological fluids and tissues as a measure of oxidant stress. Methods Enzymol. 300, 3–12.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc.,Totowa, NJ 2003

Authors and Affiliations

  • L. Jackson RobertsII
    • 1
  • Jason D. Morrow
    • 2
  1. 1.Departments of Pharmacology and MedicineVanderbilt UniversityNashville
  2. 2.Departments of Medicine and PharmacologyVanderbilt UniversityNashville

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