Analysis of F2-Isoprostanes by Gas Chromatography-Mass Spectrometry/Negative Ion Chemical Ionization
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Numerous methods have been developed to assess oxidative stress/injury in vivo in humans, which include products of oxidation of lipids, proteins, and DNA. However, it has long been recognized that most methods are unreliable because they measure products that are not specific products of free radical-induced oxidation, because the method is not specific for the oxidation product being measured, or because the method is too invasive for use in humans (1). Thus, it has long been recognized that there has been a need for a reliable method to assess oxidative stress status in vivo in humans.
KeywordsOxidative Stress Status Phosphomolybdic Acid Glass Centrifuge Tube Glass Scintillation Vial Pentafluorobenzyl Bromide
- 2.Morrow, J. D., Hill, K. E., Burk, R. F., Nammour, T. M., Badr, K. F., and Roberts, L. J. II (1990) A series of prostaglandin F2-like compounds are produced in vivos in humans by a non-cyclooxygenase free radical catalyzed mechanism. Proc. Natl. Acad. Sci. USA 87, 9383–9387.PubMedCrossRefGoogle Scholar