Single-Cell Gel Electrophoresis or Comet Assay of Intestinal Epithelial Cells Using Manual Scoring and Ridit Analysis
- 564 Downloads
The single-cell gel electrophoresis or comet assay was initially reported in 1984 as a novel technique to directly visualize DNA damage in individual cells (1). The method was modified by adding strong alkaline conditions to denature genomic DNA and detect alkali labile lesions, greatly improving sensitivity for single-strand breaks (2,3). This simple assay has come into widespread use because of its sensitivity, need for few cells, applicability to nearly all eukaryotic cells, and ease in processing numerous samples (4).
KeywordsComet Assay Comet Tail Ethidium Bromide Solution Comet Tail Length Ridit Analysis
- 5.Fleiss, J. L. (1981) The comparison of proportions from several independent samples: ridit analysis, in Statistical Methods for Rates and Proportions, 2nd ed. John Wiley & Sons, New York, pp. 150–157.Google Scholar
- 7.Tice, R. R. (1995) The single cell/gel comet assay: a microgel electrophoretic technique for the detection of DNA damage and repair in individual cells, in Environmental Mutagenesis. (Phillips, D. H. and Venitt, S., eds.) Bios Scientific Publishers, Oxford, pp. 315–339.Google Scholar