Gas Chromatography-Mass Spectrometric Analysis of Free 3-Chlorotyrosine, 3-Bromotyrosine, Ortho-Tyrosine, and 3-Nitrotyrosine in Biological Fluids
Reactive oxidants are thought to protect the host by destroying invading microbes (1). However, owing to their indiscriminate nature, reactive oxidants might also damage the host if generated inappropriately. Indeed, they have been implicated in a wide array of pathologies (2, 3, 4).
KeywordsTyrosine Microbe Phenylalanine Azide DTPA
- 5.Heinecke, J. W., Hsu, F. F., Crowley, J. R., Hazen, S. L., Leeuwenburgh, C., Mueller, D. M., et al. (1999) Detecting oxidative modification of biomolecules with isotope dilution mass spectrometry: sensitive and quantitative assays for oxidized amino acids in proteins and tissues. Methods Enzymol. 300, 124–134.PubMedCrossRefGoogle Scholar
- 7.Gaut, J. P., Byun, J., Tran, H. D., and Heinecke, J. W. (2002) Artifact-free quantification of free 3-chlorotyrosine, 3-bromotyrosine, and 3-nitrotyrosine in human plasma by electron capture-negative chemical ionization gas chromatography mass spectrometry and liquid chromatography-electrospray ionization tandem mass spectrometry. Anal. Biochem. 300, 252–259.PubMedCrossRefGoogle Scholar