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Studies of Cerebral Endothelium by Scanning and High-Voltage Electron Microscopy

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The Blood-Brain Barrier

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 89))

Abstract

This chapter reviews methods for the preparation of central nervous system (CNS) tissues for scanning (SEM) and high-voltage electron microscopic (HVEM) studies of the cerebral vasculature. The techniques described here have been used by the authors for the past two decades for investigations of pathologic alterations of the blood-brain barrier (BBB) after a variety of experimentally induced injuries in mammalian (1–4) and non-mammalian species (5–7). The question of when to use one morphologic application over another becomes important for studies of cerebrovascular endothelial cells (ECs).

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Author information

Authors and Affiliations

  1. Neural Engineering Laboratory, Huntington Medical Research Institute, Pasadena, CA

    Albert S. Lossinsky

  2. Department of Zoology, University of Western Ontario, London, Canada

    Richard R. Shivers

Authors
  1. Albert S. Lossinsky
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  2. Richard R. Shivers
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Editor information

Editors and Affiliations

  1. Division of Neuropathology, University of Toronto, Toronto, Canada

    Sukriti Nag

  2. Toronto Western Research Institute and Department of Pathology, University Health Network, Toronto, Canada

    Sukriti Nag

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© 2003 Humana Press Inc., Totowa, NJ

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Lossinsky, A.S., Shivers, R.R. (2003). Studies of Cerebral Endothelium by Scanning and High-Voltage Electron Microscopy. In: Nag, S. (eds) The Blood-Brain Barrier. Methods in Molecular Medicine™, vol 89. Humana Press. https://doi.org/10.1385/1-59259-419-0:67

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  • DOI: https://doi.org/10.1385/1-59259-419-0:67

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-073-1

  • Online ISBN: 978-1-59259-419-1

  • eBook Packages: Springer Protocols

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