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mRNA Detection in Cerebral Vessels by Nonradioactive In Situ Hybridization

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Book cover The Blood-Brain Barrier

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 89))

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Abstract

In situ hybridization (ISH) has become a critical tool for studying gene expression in the central nervous system (CNS) (1–6). ISH has advantages over immunohisto-chemistry, because ISH identifies the cells that make the antigen of interest, rather than just contain it. ISH has been used to assess the cellular distribution of messenger ribonucleic acids (mRNAs) encoding many important proteins during neural embryogenesis, and in reactive and neoplastic pathological conditions in the brain (6–10). In recent years, the sensitivity of ISH techniques has increased, using both radioactive and nonradioactive probes to allow the detection of mRNAs that encode various molecules (3,5,6,10–17) and there is increasing use of nonradioactive ISH to identify a number of genes related to vasculogenesis and angiogenesis in the brain (18–24).

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Author information

Authors and Affiliations

  1. Department of Pathology and Neurosurgery, New York University School of Medicine, New York, NY

    David Zagzag & Wai Chan

Authors
  1. David Zagzag
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  2. Wai Chan
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Editor information

Editors and Affiliations

  1. Division of Neuropathology, University of Toronto, Toronto, Canada

    Sukriti Nag

  2. Toronto Western Research Institute and Department of Pathology, University Health Network, Toronto, Canada

    Sukriti Nag

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© 2003 Humana Press Inc., Totowa, NJ

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Zagzag, D., Chan, W. (2003). mRNA Detection in Cerebral Vessels by Nonradioactive In Situ Hybridization. In: Nag, S. (eds) The Blood-Brain Barrier. Methods in Molecular Medicine™, vol 89. Humana Press. https://doi.org/10.1385/1-59259-419-0:451

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  • DOI: https://doi.org/10.1385/1-59259-419-0:451

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-073-1

  • Online ISBN: 978-1-59259-419-1

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