Abstract
The brain requires an adequate and constant supply of glucose or alternate energy substrates to support its metabolic demands. Because of the special conditions imposed by the presence of the blood-brain barrier (BBB), specific transport mechanisms are required for the influx of water-soluble substrates. Alterations in energy substrate transport or availability has profound consequences that may result in inadequate energy supply and possible cell death. To study these transport mechanisms, quantitative methods of substrate influx and blood flow have been developed. These methods are based on a model of unidirectional tracer influx at the endothelial cell boundary from the blood during capillary transit (1).
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Puchowicz, M.A., Xu, K., LaManna, J.C. (2003). Single-Pass Dual-Label Indicator Method. In: Nag, S. (eds) The Blood-Brain Barrier. Methods in Molecular Medicine™, vol 89. Humana Press. https://doi.org/10.1385/1-59259-419-0:265
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DOI: https://doi.org/10.1385/1-59259-419-0:265
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