Abstract
The blood-brain barrier (BBB) serves to regulate passage of solutes and water between circulating blood and brain extracellular fluid (1,2). It is composed of endothelial cells that line brain capillaries and exhibit selective transport properties. Cerebral endothelial cells are unusual in that they possess true tight junctions (zonula occludens), which provide a relatively high electrical resistance, and impart polarity to the plasma membrane (3,4). The presence of tight junctions limits the movement of solutes through the intercellular space, and separates the plasmalemma into luminal (blood-facing) and abluminal (brain-facing) plasma membrane domains. Thus, penetration of the BBB is for the most part transcellular, and requires transport across the respective plasma membrane domains (5,6).
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References
Betz, A. L. (1986) Transport of ions across the blood-brain barrier. Fed. Proc. 45, 2050ā2054.
Betz, A. L., and Goldstein, G. W. (1986) Specialized properties and solute transport in brain capillaries. Ann. Rev. Physiol. 48, 241ā250.
Betz, A. L., Firth, J. A., and Goldstein, G. W. (1980) Polarity of the blood-brain barrier: Distribution of enzymes between the luminal and antiluminal membranes of brain capillary endothelial cells. Brain Res. 192,17ā28.
Reese, T S., and Karnovsky, M. J. (1967) Fine structural localization of a blood-brain barrier to exogenous peroxidase. J. Cell Biol. 34, 207ā217.
Brightman, M. W., and Reese, T. W. (1969) Junctions between intimately apposed cell membranes in the vertebrate brain. J. Cell Biol. 40, 648ā677.
Pardridge, W. M. (1983) Brain metabolism: a perspective from the blood-brain barrier. Physiol. Rev. 63,1481ā1535.
Betz, A. L., and Goldstein, G W. (1978) Polarity of the blood-brain barrier: Neutral amino acid transport into isolated brain capillaries. Science 202, 225ā226.
Pardridge, W. M., Eisenberg, J. and Yamada, T (1985) Rapid sequestration and degradation of somatostatin analogues by isolated brain microvessels. J. Neurochem. 44,1178ā1184.
Peterson, D. R. and Hawkins, R. A. (1998) Isolation and behavior of plasma membrane vesicles made from cerebral capillary endothelial cells. In Introduction to the Blood-Brain Barrier. Pardridge, W. M., ed. Cambridge University Press, London, pp. 62ā70.
Sanchez del Pino, M. M., Hawkins, R. A., and Peterson, D. R. (1992) Neutral amino acid transport by the blood-brain barrier: Membrane vesicle studies. J. Biol. Chem. 267, 25951ā25957.
Sanchez del Pino, M. M., Hawkins, R. A., and Peterson, D. R. (1995) Biochemical discrimination between luminal and abluminal enzyme and transport activities of the blood-brain barrier. J. Biol. Chem. 270,14907ā14912.
Peterson, D. R., Rambow, J., Sukowski, E. J., and Zikos, D. (1999) Glutathione transport by the blood-brain barrier. FASEB J. 13, A709.
Sanchez del Pino, M. M. (1994) Neutral amino acid transport by the blood-brain barrier using isolated membrane vesicles. Ph.D. Thesis. Finch University of Health Sciences/The Chicago Medical School, N. Chicago, IL.
Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248ā254.
Sanchez del Pino, M. M., Peterson, D. R., and Hawkins, R. A. (1995) Neutral amino acid transport characterization of isolated luminal and abluminal membranes of the blood-brain barrier. J. Biol. Chem. 270, 14913ā14918.
Lee, W-J., Hawkins, R. A., Peterson, D. R., and Vina, J. (1996) Role of oxoproline in the regulation of neutral amino acid transport across the blood-brain barrier. J. Biol. Chem. 271, 19129ā19133.
Lee, W-J., Hawkins, R. A., Vina, J. R. and Peterson, D. R. (1998) Glutamine transport by the blood-brain barrier: a possible mechanism for nitrogen removal. Am. J. Physiol. 274, C1101āC1107.
Lee, W-J., Peterson, D. R., Sukowski, E. J., and Hawkins, R. A. (1997) Glucose transport by isolated plasma membranes of the blood-brain barrier. Am. J. Physiol. 272, C1552āC1557.
Peterson, D. R., Rambow, J., Sukowski, E. J., and Zikos, D. (2000) Mechanisms for sodium transport by the blood-brain barrier. FASEB J. 14, LB74.
Skopicki, H. A., Fisher, K., Zikos, D., Flouret, G., and Peterson, D. R. (1989) Low-affinity transport of pyroglutamyl-histidine in renal brush-border membrane vesicles. Am. J. Physiol. 257, C971āC975.
Hopfer, U. (1989) Tracer studies with isolated membrane vesicles. Methods Enzymol. 172, 313ā321.
Malo, C, and Berteloot, A. (1991) Analysis of kinetic data in transport studies: new insights from kinetic studies of Na+-D-glucose cotransport in human intestinal brush-border membrane vesicles using a fast sampling, rapid filtration apparatus. J. Membr. Biol. 122,127ā141.
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Peterson, D.R., Hawkins, R.A. (2003). Transport Studies Using Membrane Vesicles. In: Nag, S. (eds) The Blood-Brain Barrier. Methods in Molecular Medicineā¢, vol 89. Humana Press. https://doi.org/10.1385/1-59259-419-0:233
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DOI: https://doi.org/10.1385/1-59259-419-0:233
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