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Gene and Enhancer Traps for Gene Discovery

  • Marcela Rojas-Pierce
  • Patricia S. Springer
Part of the Methods in Molecular Biology™ book series (MIMB, volume 236)

Abstract

Gene traps and enhancer traps provide a valuable tool for gene discovery. With this system, genes can be identified based solely on the expression pattern of an inserted reporter gene. The use of a reporter gene, such as β-glucuoronidase (GUS), provides a very sensitive assay for the identification of tissue- and cell-type specific expression patterns. In this chapter, protocols for examining and documenting GUS reporter gene activity in individual lines are described. Methods for the amplification of sequences flanking transposant insertions and subsequent molecular and genetic characterization of individual insertions are provided.

Key Words

Arabidopsis Ds gene trap enhancer trap transposable element GUS gene expression TAIL-PCR transposon tagging mutagenesis 

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Copyright information

© Humana Press Inc., Totowa, NJ 2003

Authors and Affiliations

  • Marcela Rojas-Pierce
    • 1
  • Patricia S. Springer
    • 1
  1. 1.Department of Botany and Plant Sciences, Center for Plant Cell BiologyUniversity of CaliforniaRiverside

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