Abstract
In this chapter, we describe the use of plasmid vectors in transcription and translation systems in vitro to investigate aspects of the biology of the gene and the protein for which it codes. An in vitro, or cell-free, assay reproduces a relatively complex physiological process by mixing the essential purified components of the system under controlled conditions outside of the cell. Such systems allow the basic steps of transcription and translation to be studied individually, and the products obtained at each step to be altered in different ways according to the needs of the research. Thus, an in vitro system is convenient when it is necessary to modify a product, for example, by introducing mutations, labels, tags, or fusions.
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Movahedzadeh, F., Rico, S.G., Cox, R.A. (2003). In Vitro Transcription and Translation. In: Casali, N., Preston, A. (eds) E. coli Plasmid Vectors. Methods in Molecular Biology™, vol 235. Humana Press. https://doi.org/10.1385/1-59259-409-3:247
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DOI: https://doi.org/10.1385/1-59259-409-3:247
Publisher Name: Humana Press
Print ISBN: 978-1-58829-151-6
Online ISBN: 978-1-59259-409-2
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