Screening Recombinant DNA Libraries

  • Wade A. Nichols
Part of the Methods in Molecular Biology™ book series (MIMB, volume 235)


A recombinant DNA library typically represents part or all of an organism’s genomic DNA or mRNA (represented as cDNA) cloned into vectors and stored as a collection of thousands of transformants. The construction of a complete library is only half the task; researchers then need to be able to identify the small number of clones bearing the DNA fragment of interest among the numerous transformants within the library. This process is called “screening a library” and it is the molecular equivalent of finding a needle in a haystack. Libraries may be screened by any one of several methods.


Library Clone Dark Spot Label Probe Wash Solution Multiwell Plate 
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Copyright information

© Humana Press Inc. 2003

Authors and Affiliations

  • Wade A. Nichols
    • 1
  1. 1.Department of Biological SciencesIllinois State UniversityNormal

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