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Screening Recombinant DNA Libraries

  • Wade A. Nichols
Part of the Methods in Molecular Biology™ book series (MIMB, volume 235)

Abstract

A recombinant DNA library typically represents part or all of an organism’s genomic DNA or mRNA (represented as cDNA) cloned into vectors and stored as a collection of thousands of transformants. The construction of a complete library is only half the task; researchers then need to be able to identify the small number of clones bearing the DNA fragment of interest among the numerous transformants within the library. This process is called “screening a library” and it is the molecular equivalent of finding a needle in a haystack. Libraries may be screened by any one of several methods.

Keywords

Library Clone Dark Spot Label Probe Wash Solution Multiwell Plate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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    Southern, E. M. (1975) Detection of specific sequences among DNA fragments separated by gel electrohoresis. J. Mol. Biol. 98, 503–517.PubMedCrossRefGoogle Scholar
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    Feinberg, A. P. and Vogelstein, B. (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132, 6–13.PubMedCrossRefGoogle Scholar
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    Denhardt, D. (1966) A membrane filter technique for the detection of complementary DNA. Biochem. Biophys. Res. Commun. 23, 641–646.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 2003

Authors and Affiliations

  • Wade A. Nichols
    • 1
  1. 1.Department of Biological SciencesIllinois State UniversityNormal

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