p53-Induced Gene Expression Analysis

  • Joanna K. Sax
  • Wafik S. El-Deiry
Part of the Methods in Molecular Biology book series (MIMB, volume 234)

Abstract

Researchers in the p53 field have successfully used many high-throughput screening technologies to analyze and characterize p53-induced gene expression. This chapter will focus on one such technology, the Affymetrix GeneChip®. DNA-Chip technology has grown rapidly over the last several years. The ability to hybridize RNA from a sample to thousands to tens of thousands of known and unknown cDNAs spotted on a microarray chip has led to the explosion of information ranging from macro-global expression pattern changes to micro-gene-specific expression changes. The relative ease of making a nonradioactive probe from either total RNA or mRNA to be hybridized to a GeneChip makes microarray technology highly attractive.

Key Words

p53 transcription microarray gene expression DNA-Chip 

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Copyright information

© Humana Press Inc., Totowa, NJ 2003

Authors and Affiliations

  • Joanna K. Sax
    • 1
    • 2
    • 3
    • 4
    • 5
  • Wafik S. El-Deiry
    • 1
    • 2
    • 3
    • 4
    • 5
  1. 1.Laboratory of Molecular Oncology and Cell Cycle Regulation, Howard Hughes Medical InstituteUniversity of Pennsylvania School of MedicinePhiladelphia
  2. 2.Department of MedicineUniversity of Pennsylvania School of MedicinePhiladelphia
  3. 3.Department of GeneticsUniversity of Pennsylvania School of MedicinePhiladelphia
  4. 4.Department of PharmacologyUniversity of Pennsylvania School of MedicinePhiladelphia
  5. 5.Cancer CenterUniversity of Pennsylvania School of MedicinePhiladelphia

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