Abstract
For the cell culturist, two types of contamination require careful monitoring and constant vigilance: the contamination of cell cultures with microbiological organisms and the contamination of one cell line with another. Both forms of contamination are extremely prevalent and cannot be underestimated. Neither type can be eliminated, only controlled and managed to minimize the possibility of occurrence. Contamination consequences can range from minor inconvenience (a flask of cells becoming contaminated with bacteria) to a major disaster (published results that may be invalid owing to cross-contamination of one cell line with another). Other types of contaminants, such as chemical contamination, may also cause problems (e.g., deposits of disinfectants or detergents on glassware; residues, impurities, and toxins in water, media or sera), but the common recurring problems are likely to be biological in origin.
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References
Rottern, S. and Barile, M. F. (1993) Beware of mycoplasmas. Trends Biotechnol. 11, 143ā151.
McGarrity, G. J., Phillips, D., and Vaidya, A. (1980) Mycoplasma infection of lymphocyte cultures: infection with M. salivarium. In vitro 16, 346ā356.
Aula, P. and Nichols, W. W. (1967) The cytogenetic effects of mycoplasma in human leucocyte cultures. J. Cell Physiol. 70, 281ā290.
Perez A. G., Kim, J. H., Gelbard, A. S., and Djordjevic, B. (1972) Altered incorporation of nucleic acid precursors by mycoplasma-infected mammalian cells in culture. Exp. Cell. Res. 70, 301ā310.
Levine, E. M., Thomas, L., McGregor, D., Hayflick, L., and Eagle, M. (1968) Altered nucleic acid metabolism in human cell cultures infected with mycoplasma. Proc. Natl. Acad. Sci. USA 60, 583ā589.
Stanbridge, E. J., Hayflick, L., and Perkins, F. T. (1971) Modification of amino acid concentrations induced by mycoplasmas in cell culture medium. Nature 232, 242ā244.
Wise, K. S., Cassell, G. H., and Action, R. T. (1978). Selective association of murine T lymphoblastoid cell surface alloantigens with mycoplasma hyorhinis. Proc. Natl. Acad. Sci. USA 75, 4479ā4483.
Denecke, J., Becker, K., Jurgens, H., Gross, R., and Wolff, J. E. (1999) Falsification of tetrazolium dye (MTT) based cytotoxicity assay results due to mycoplasma contamination of cell cultures. Anticancer Res. 19, 1245ā1248.
Kappeler, A., Lutz-Wallace, C., Sapp, T., and Sidhu, M. (1996) Detection of bovine contamination in fetal bovine sera and modified live viral vaccine using polymerase chain reaction. Biologicals 24, 131ā135.
Nelson-Rees, W. A. (2001) Responsibility for truth in research. Philos. Trans. R. Soc. Lond. B Biol. Sci. 356, 849ā851.
Gartler, S. M. (1968) Apparent HeLa contamination of human heterodiploid cell lines. Nature 217, 750ā751.
Nelson-Rees, W. A., Flandermeyer, R. R., and Hawthorne P. K. (1974) Banded marker chromosomes as indicators of intraspecies cellular contamination. Science 184, 1093ā1096.
Nelson-Rees, W. A. and Flandermeyer, R. R. (1976) HeLa cultures defined. Science 191, 96ā98.
Nelson-Rees, W. A., Daniels, D. W., and Flandermeyer, R. R. (1981) Cross-contamination of cells in culture. Science 212, 446ā452.
Nelson-Rees, W. A., and Flandermeyer, R. R. (1977) Inter-and intraspecies contamination of human breast tumor cell lines HBC and BrCa5 and other cell cultures HeLa cultures defined. Science 195, 1343ā1344.
Stulberg, C. S., Peterson, W. D., and Simpson, W. F. (1976) Identification of cells in culture. Am. J. Hematol. 1, 237ā242.
Hukku, B., Halton, D. M., Mally, M., and Peterson, W. D. (1984) Cell characterization by the use of multiple genetic markers. Adv. Exp. Med. Biol. 172, 13ā31.
MacLeod, R. A. F., Dirks, W. G., Matsuo, Y., Kaufmann, M., Milch, H., and Drexler, H. G. (1999) Widespread intraspecies cross-contamination of human tumor cell lines arising at source. Int. J. Cancer 83, 555ā563.
Stacey, G. N., Masters, J. R. W., Hay, R. J., Drexler, H. G., MacLeod, R. A. F., and Freshney, R. I. (2000) Cell contamination leads to inaccurate data: we must take action now. Nature 403, 356.
MacLeod, R. A. F., Dirks, W. G., and Drexler, H. G. (2002) Persistent use of misidentified cell lines and its prevention. Genes Chromosomes Cancer 33, 103ā105.
UKCCCR (2000). UKCCCR Guidelines for the use of cell lines in cancer research. Brit. J. Cancer 82, 1495ā1509.
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Ā© 2004 Humana Press Inc., Totowa, NJ
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Langdon, S.P. (2004). Cell Culture Contamination. In: Langdon, S.P. (eds) Cancer Cell Culture. Methods in Molecular Medicineā¢, vol 88. Humana Press. https://doi.org/10.1385/1-59259-406-9:309
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DOI: https://doi.org/10.1385/1-59259-406-9:309
Publisher Name: Humana Press
Print ISBN: 978-1-58829-079-3
Online ISBN: 978-1-59259-406-1
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