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The Use of Matrigel to Facilitate the Establishment of Human Cancer Cell Lines as Xenografts

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Cancer Cell Culture

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 88))

Abstract

Matrigel is a solubilized tissue basement membrane matrix rich in extracellular matrix proteins that was originally isolated from the Engelbreth-Holm-Swarm (EHS) mouse tumor. Although composed mainly of laminin, collagen IV, heparan sulphate, proteoglycans, and entactin (nidogen), various growth factors such as transforming growth factor-β (TGF-β), fibroblast growth factor (FGF), and tissue plasminogen activator are also present. Under normal physiological conditions Matrigel polymerizes to produce a reconstituted, biologically active stable matrix, that is effective for the attachment and differentiation of normal and transformed anchorage-dependent cell types (1–2).

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Authors and Affiliations

  1. Cancer Research UK Oncology Unit, Western General Hospital, Edinburgh, UK

    Peter Mullen

Authors
  1. Peter Mullen
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Editor information

Editors and Affiliations

  1. Western General Hospital Edinburgh, UK

    Simon P. Langdon

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© 2004 Humana Press Inc., Totowa, NJ

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Mullen, P. (2004). The Use of Matrigel to Facilitate the Establishment of Human Cancer Cell Lines as Xenografts. In: Langdon, S.P. (eds) Cancer Cell Culture. Methods in Molecular Medicine™, vol 88. Humana Press. https://doi.org/10.1385/1-59259-406-9:287

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  • DOI: https://doi.org/10.1385/1-59259-406-9:287

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-079-3

  • Online ISBN: 978-1-59259-406-1

  • eBook Packages: Springer Protocols

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