Abstract
Transfection is the process by which exogenous DNA is transferred into eukaryotic cells. This allows the functional study of a specific gene product within a cellular context. To facilitate expression of the gene of interest, it is first cloned into a vector plasmid DNA that supplies necessary transcriptional and translational control elements (see Fig. 1). There are two types of transfection; transient transfection and stable transfection. Both have advantages and limitations.
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© 2004 Humana Press Inc., Totowa, NJ
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O’ Donnell, A.J.M. (2004). DNA-Mediated Gene Transfer. In: Langdon, S.P. (eds) Cancer Cell Culture. Methods in Molecular Medicine™, vol 88. Humana Press. https://doi.org/10.1385/1-59259-406-9:259
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DOI: https://doi.org/10.1385/1-59259-406-9:259
Publisher Name: Humana Press
Print ISBN: 978-1-58829-079-3
Online ISBN: 978-1-59259-406-1
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