Purification of Membrane-Bound Catechol-O-Methyltransferase

  • Maria João Bonifácio
  • Patrício Soares-da-Silva
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 228)

Abstract

Catechol-0-methyltransferase (COMT, EC 2.1.1.6) was first detected in rat liver extracts (1) and since then, COMT has been found in plants, yeast, invertebrates, and vertebrates. In mammals, the highest COMT activities are encountered in liver, kidney, and gut wall (2,3). The general physiological function of COMT is the inactivation of biologically active or toxic catechols. The enzyme catalyzes the transference of a methyl group from S-adenosyl-l-methionine (SAM) to a hydroxyl group on a catechol substrate in the presence of magnesium and the reaction products are the O-methylated catechol and S-adenosyl-l-homocysteine (1).

Keywords

HPLC Magnesium Hydroxyl Estrogen Ethylene Glycol 

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Copyright information

© Humana Press Inc., Totowa, NJ 2003

Authors and Affiliations

  • Maria João Bonifácio
    • 1
  • Patrício Soares-da-Silva
    • 1
  1. 1.Department of Research and DevelopmentBIALPortugal

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