Abstract
As protein kinase C (PKC) isozymes are implicated in increasing numbers of signal transduction pathways and disease processes, more investigators from different fields have the need to assay PKC activity. A simple in vitro assay suitable for measuring PKC activity in soluble cell extracts is provided here. Like other kinases, PKC catalyzes the transfer of the terminal phosphate from ATP to a protein or peptide substrate. Use of γ-32P-labeled ATP allows this transfer to be monitored by scintillation counting or by autoradiography of the substrate following its removal from the excess ATP.
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© 2003 Humana Press Inc., Totowa, NJ
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Sando, J.J., Beals, J.K. (2003). Enzyme Assays for Protein Kinase C Activity. In: Newton, A.C. (eds) Protein Kinase C Protocols. Methods in Molecular Biology™, vol 233. Humana Press. https://doi.org/10.1385/1-59259-397-6:63
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DOI: https://doi.org/10.1385/1-59259-397-6:63
Publisher Name: Humana Press
Print ISBN: 978-1-58829-068-7
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