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Protein Kinase C Protocols pp 455–473Cite as

Animal Models in the Study of Protein Kinase C Isozymes

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 233))

Abstract

The decoding of genomes for several species has provided investigators with an unprecedented wealth of DNA sequence information to identify, mutate, and overexpress genes in animals, and study phenotypes that result from these manipulations. This is particularly valuable for the study of the protein kinase C (PKC) family because the pharmacological tools available to examine individual PKC isozymes in whole animals are few. This chapter reviews some of the animal models and approaches that have been used and discusses some of the limitations inherent in these studies, particularly those involving knockout and transgenic mice. A protocol for identifying founder lines of transgenic mice that express the tetO-minimal CMV (Ptet) promoter driving a mouse PKCε cDNA transgene is also described.

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Authors and Affiliations

  1. Department of Neurology, Ernest Gallo Clinic and Research Center, University of California San Francisco, Emeryville, CA

    Doo-Sup Choi & Robert O. Messing

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  1. Doo-Sup Choi
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  2. Robert O. Messing
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  1. Department of Pharmacology, University of California at San Diego, La Jolla, CA

    Alexandra C. Newton

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© 2003 Humana Press Inc., Totowa, NJ

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Choi, DS., Messing, R.O. (2003). Animal Models in the Study of Protein Kinase C Isozymes. In: Newton, A.C. (eds) Protein Kinase C Protocols. Methods in Molecular Biology™, vol 233. Humana Press. https://doi.org/10.1385/1-59259-397-6:455

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  • DOI: https://doi.org/10.1385/1-59259-397-6:455

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-068-7

  • Online ISBN: 978-1-59259-397-2

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