Enzyme Assays for Protein Kinase C Activity

  • Julianne J. Sando
  • Jacquelyn K. Beals
Part of the Methods in Molecular Biology™ book series (MIMB, volume 233)


As protein kinase C (PKC) isozymes are implicated in increasing numbers of signal transduction pathways and disease processes, more investigators from different fields have the need to assay PKC activity. A simple in vitro assay suitable for measuring PKC activity in soluble cell extracts is provided here. Like other kinases, PKC catalyzes the transfer of the terminal phosphate from ATP to a protein or peptide substrate. Use of γ-32P-labeled ATP allows this transfer to be monitored by scintillation counting or by autoradiography of the substrate following its removal from the excess ATP.


Phorbol Ester Assay Tube Geiger Counter Cerenkov Counting Soluble Cell Extract 
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Copyright information

© Humana Press Inc., Totowa, NJ 2003

Authors and Affiliations

  • Julianne J. Sando
    • 1
  • Jacquelyn K. Beals
    • 1
  1. 1.Department of AnesthesiologyUniversity of VirginiaCharlottesville

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