Enzyme Assays for Protein Kinase C Activity

  • Julianne J. Sando
  • Jacquelyn K. Beals
Part of the Methods in Molecular Biology™ book series (MIMB, volume 233)

Abstract

As protein kinase C (PKC) isozymes are implicated in increasing numbers of signal transduction pathways and disease processes, more investigators from different fields have the need to assay PKC activity. A simple in vitro assay suitable for measuring PKC activity in soluble cell extracts is provided here. Like other kinases, PKC catalyzes the transfer of the terminal phosphate from ATP to a protein or peptide substrate. Use of γ-32P-labeled ATP allows this transfer to be monitored by scintillation counting or by autoradiography of the substrate following its removal from the excess ATP.

Keywords

Vortex Hydrate Argon Chloroform Electrophoresis 

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Copyright information

© Humana Press Inc., Totowa, NJ 2003

Authors and Affiliations

  • Julianne J. Sando
    • 1
  • Jacquelyn K. Beals
    • 1
  1. 1.Department of AnesthesiologyUniversity of VirginiaCharlottesville

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