Methods for Detecting Binding Proteins

An Introduction
  • Gerda Endemann
  • Daria Mochly-Rosen
Part of the Methods in Molecular Biology™ book series (MIMB, volume 233)

Abstract

The identification of proteins that bind protein kinase C (PKC) is essential to understanding the contributions of PKCs to signaling pathways. A number of the methods are uniquely applicable to this family of serine/threonine kinases. The PKC family contains at least 10 closely related members with overlapping substrate specificities (1,2). Therefore, isozyme-specific phosphorylation of substrates must be caused in part by isozyme-specific subcellular localization, isozyme-specific proximity to substrates, and/or to regulation of activity in an isozyme-specific manner (3). There is some selectivity in the regulation of catalytic activity of PKC classes rather than individual isozymes. The classic (cPKC) enzymes α, βI, βII, and γPKC are activated by phosphatidylserine (PS), diacylglycerol (DG), and calcium, the novel (nPKC) enzymes δ, ε, θ, and ηPKC require PS and DG only, and the atypical (aPKC) enzymes ζ, and λ/ιPKC are activated by PS and possibly phosphatidylinositol-3,4,5-trishosphate, and ceramide (1,4,5).

Keywords

Tyrosine Glutathione Influenza Fractionation Immobilization 

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Copyright information

© Humana Press Inc., Totowa, NJ 2003

Authors and Affiliations

  • Gerda Endemann
    • 1
  • Daria Mochly-Rosen
    • 1
  1. 1.Department of Molecular PharmacologyStanford University School of MedicineStanford

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