Abstract
As delineated in Chapter 26, the directed evolution of enantioselective enzymes for use in synthetic organic chemistry requires the availability of high-throughput screening systems for determining the enantiomeric excess (ee) of thousands of samples (1). In this chapter the protocols of some of the most practical ee-screens are presented.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Reetz, M. T. (2000) Application of directed evolution in the development of enantioselective enzymes. Pure Appl. Chem. 72, 1615–1622.
Reetz, M. T. and Jaeger, K.-E. (2000) Enantioselective enzymes for organic synthesis created by directed evolution. Chem. Eur. J. 6, 407–412.
Reetz, M. T. and Jaeger, K.-E. (2002) Directed evolution as a means to create enantioselective enzyme for use in organic chemistry, in Directed Molecular Evolution of Proteins (Brakmann, S. and Johnson, K., eds.), Wiley-VCH Verlag GmbH, Weinheim, Germany, pp. 245–279.
Reetz, M. T., Becker, M. H., Klein, H.-W., and Stöckigt, D. (1999) A method for high-throughput screening of enantioselective catalysts. Angew. Chem. 111, 1872–1875; Angew. Chem. Int. Ed. Engl. 38, 1758–1761.
Schrader, W., Eipper, A., Pugh, D. J., and Reetz, M. T. (2002) Second-generation MS-based high-throughput screening system for enantioselective catalysts and biocatalysts. Can. J. Chem. 80, 626–632.
Chen, C.-S., Fujimoto, Y., Girdaukas, G., and Sih, C. J. (1982) Quantitative analyses of biochemical kinetic resolutions of enantiomers. J. Am. Chem. Soc. 104, 7294–7299.
Kagan, H. B. and Fiaud, J. C. (1988) Kinetic resolution. Top. Stereochem. 18, 249–330.
Reetz, M. T., Eipper, A., Tielmann, P., and Mynott, R. (2002) A Practical NMR-based high-throughput assay for screening enantioselective catalysts and biocatalysts. Adv. Synth. Catal., in press.
Reetz, M. T., Eipper, A., Tielmann, P., and Mynott, R. (2002) Studiengesellschaft Kohle mbH. Patent application no. DE-A 102 09 177.3.
Reetz, M. T., Kühling, K. M., Wilensek, S., Husmann, H., Häusig, U. W., and Hermes, M. (2001) A GC-based method for high-throughput screening of enantioselective catalysts. Catal. Today 67, 389–396.
Ross, A., Schlotterbeck, G., Senn, H., and von Kienlin, M. (2001) Application of chemical shift imaging for simultaneous and fast acquisition of NMR spectra on multiple samples. Angew. Chem. 113, 3343–3345; Angew. Chem. Int. Ed. Engl. 40, 3243–3245.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2003 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Reetz, M.T. (2003). Select Protocols of High-Throughput ee-Screening Systems for Assaying Enantioselective Enzymes. In: Arnold, F.H., Georgiou, G. (eds) Directed Enzyme Evolution. Methods in Molecular Biology™, vol 230. Humana Press. https://doi.org/10.1385/1-59259-396-8:283
Download citation
DOI: https://doi.org/10.1385/1-59259-396-8:283
Publisher Name: Humana Press
Print ISBN: 978-1-58829-286-5
Online ISBN: 978-1-59259-396-5
eBook Packages: Springer Protocols