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Select Protocols of High-Throughput ee-Screening Systems for Assaying Enantioselective Enzymes

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Directed Enzyme Evolution

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 230))

Abstract

As delineated in Chapter 26, the directed evolution of enantioselective enzymes for use in synthetic organic chemistry requires the availability of high-throughput screening systems for determining the enantiomeric excess (ee) of thousands of samples (1). In this chapter the protocols of some of the most practical ee-screens are presented.

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References

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© 2003 Humana Press Inc., Totowa, NJ

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Reetz, M.T. (2003). Select Protocols of High-Throughput ee-Screening Systems for Assaying Enantioselective Enzymes. In: Arnold, F.H., Georgiou, G. (eds) Directed Enzyme Evolution. Methods in Molecular Biology™, vol 230. Humana Press. https://doi.org/10.1385/1-59259-396-8:283

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  • DOI: https://doi.org/10.1385/1-59259-396-8:283

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-286-5

  • Online ISBN: 978-1-59259-396-5

  • eBook Packages: Springer Protocols

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