Abstract
Oxidases remove hydrogen from substrates, transferring it to molecular oxygen to generate hydrogen peroxide or water. Since oxidases catalyze fundamental synthetic transformations, including alcohol-aldehyde and aldehyde-carboxylic acid conversions, they have attracted attention as targets of directed evolution for further improvement (1,2). The co-product hydrogen peroxide can be detected by various methods (3) and provides a basis for screening oxidases in a high throughput fashion.
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© 2003 Humana Press Inc., Totowa, NJ
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Sun, L., Yagasaki, M. (2003). Screen for Oxidases by Detection of Hydrogen Peroxide with Horseradish Peroxidase. In: Arnold, F.H., Georgiou, G. (eds) Directed Enzyme Evolution. Methods in Molecular Biology™, vol 230. Humana Press. https://doi.org/10.1385/1-59259-396-8:177
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DOI: https://doi.org/10.1385/1-59259-396-8:177
Publisher Name: Humana Press
Print ISBN: 978-1-58829-286-5
Online ISBN: 978-1-59259-396-5
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