Abstract
SCRATCHY is a combination of the incremental truncation for the creation of hybrid enzymes (ITCHY) technology (1) and DNA shuffling (2). It generates combinatorial libraries of hybrid proteins consisting of multiple fragments from two or more parental DNA sequences with no restriction to DNA sequence identity between the original sequences (3). Such multi-crossover hybrids can be of interest to the studies of fundamental questions of protein evolution and folding, as well as to the tailoring of enzymes for therapeutic and industrial applications.
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References
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© 2003 Humana Press Inc., Totowa, NJ
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Lutz, S., Ostermeier, M. (2003). Preparation of SCRATCHY Hybrid Protein Libraries. In: Arnold, F.H., Georgiou, G. (eds) Directed Evolution Library Creation. Methods in Molecular Biology™, vol 231. Humana Press. https://doi.org/10.1385/1-59259-395-X:143
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DOI: https://doi.org/10.1385/1-59259-395-X:143
Publisher Name: Humana Press
Print ISBN: 978-1-58829-285-8
Online ISBN: 978-1-59259-395-8
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