Abstract
Overexpression and characterization of mutant proteins in mammalian cells is of crucial importance in order to understand the disease-causing nature of mutant proteins in human diseases. Much of the present knowledge of the mechanisms behind disease-causing proteins comes from cellular analysis of the plethora of naturally occurring mutations. Functional testing of these gene variants depends on efficient gene transfer to mammalian cells. A wide variety of methods for transfection of tissue-culture cells has been applied. The two common methods for transient transfection of adherent cells, chemical treatment with calcium-phosphate and lipid-based procedures, have all been used and will be described here. For a detailed treatment of the subject, including stable transfection and viral-mediated gene transfer, the reader is referred to refs. 1–6.
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Corydon, T.J. (2003). Characterization of Overexpressed Mutant Proteins in Mammalian Cells. In: Bross, P., Gregersen, N. (eds) Protein Misfolding and Disease. Methods in Molecular Biology™, vol 232. Humana Press. https://doi.org/10.1385/1-59259-394-1:183
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DOI: https://doi.org/10.1385/1-59259-394-1:183
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