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Cells of Respiratory Epithelium

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Lentivirus Gene Engineering Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 229))

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Abstract

The goal of correcting such genetic lung diseases as cystic fibrosis could be achieved by vector-mediated gene transfer to airway epithelia. The principle of using viral vectors to complement genetic defects is well documented in cell culture and in animal models. Moreover, strategies for the successful implementation of gene transfer-based therapies for patients are beginning to emerge (1). However, the airway epithelium has evolved many defensive barriers against microbial invasion (2,3). Indeed, these same barriers likely impede gene transfer efficiency with viral and nonviral vectors (3).

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Author information

Authors and Affiliations

  1. Program in Gene Therapy, Department of Pediatrics, University of Iowa, College of Medicine, Iowa City, IA

    Patrick L. Sinn

  2. Department of Pediatrics, University of Iowa, College of Medicine, Iowa City, IA

    Paul B. McCray Jr.

Authors
  1. Patrick L. Sinn
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  2. Paul B. McCray Jr.
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Editor information

Editors and Affiliations

  1. Laboratory of Virology, Istituto Superiore di Sanitá, Rome, Italy

    Maurizio Federico

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© 2003 Humana Press Inc.

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Sinn, P.L., McCray, P.B. (2003). Cells of Respiratory Epithelium. In: Federico, M. (eds) Lentivirus Gene Engineering Protocols. Methods in Molecular Biology™, vol 229. Humana Press. https://doi.org/10.1385/1-59259-393-3:287

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  • DOI: https://doi.org/10.1385/1-59259-393-3:287

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-091-5

  • Online ISBN: 978-1-59259-393-4

  • eBook Packages: Springer Protocols

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