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Lentivirus Gene Engineering Protocols pp 273–284Cite as

Hybrid Lentivirus Vectors

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 229))

Abstract

Lentivirus vectors are promising tools for gene transfer (1,2). Retroviral vectors in general offer the unique advantage of stably integrating into the genome of the host cell, thus providing the basis for sustained gene expression. In contrast to the classical oncoretrovirus-derived vectors, lentivirus vectors are highly efficient at infection of nondividing cells. This unique ability is owing to the presence of nuclear localization signals on several virion associated proteins, which include matrix (MA), viral protein R (VPR), and integrase (IN) (3) and a cis-acting element termed the central polypurine tract (cPPT) (4,5).

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Author information

Authors and Affiliations

  1. School of Medicine, University of Utah, Salt Lake City, UT

    Vicente Planelles

Authors
  1. Vicente Planelles
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Editor information

Editors and Affiliations

  1. Laboratory of Virology, Istituto Superiore di Sanitá, Rome, Italy

    Maurizio Federico

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© 2003 Humana Press Inc.

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Planelles, V. (2003). Hybrid Lentivirus Vectors. In: Federico, M. (eds) Lentivirus Gene Engineering Protocols. Methods in Molecular Biology™, vol 229. Humana Press. https://doi.org/10.1385/1-59259-393-3:273

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  • DOI: https://doi.org/10.1385/1-59259-393-3:273

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-091-5

  • Online ISBN: 978-1-59259-393-4

  • eBook Packages: Springer Protocols

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