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Laser-Capture Microdissection

  • Protocol
Renal Disease

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 86))

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Abstract

The kidney is an anatomically complex organ with exceptional cellular heterogeneity. Our understanding of renal physiology has been advanced by studies of hand-dissected individual tubules (microdissection). Glomeruli or renal tubules are dissected away from surrounding structures, and subjected to microassays for enzymatic activity and receptor function, in vitro micro-perfusion for transport rates, and single-tubule reverse transcriptase-polymerase chain reaction (RT-PCR) (1). Thus, microdissection methods have allowed assay of activity and mRNA and protein expression in single-nephron segments in normal or uninjured kidneys. Renal disease may be global, but more typically involves selective injury to the glomerulus, portions of the nephron, interstitium, or blood vessels. Less is known about renal pathophysiology and the nephron-specific response to injury. For example, how do different portions of the nephron interact during renal injury? Why are some renal diseases focal? How do individual glomeruli react in focal semental glomerulo-sclerosis (FSGS)? Does the interaction tend to propagate or to defend against further injury? Microdissection techniques have not been widely used to study renal injury because microdissection is often limited by tissue necrosis or fibrosis. In addition, the time required for microdissection often exceeds the time-course of rapid and transient changes in a cellular response such as certain metabolic intermediates or early-response genes.

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Authors and Affiliations

  1. National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD

    Laura Barisoni & Robert A. Star

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  1. Laura Barisoni
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  2. Robert A. Star
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  1. New York Medical College, Valhalla, NY

    Michael S. Goligorsky MD, PhD

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© 2003 Humana Press Inc., Totowa, NJ

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Barisoni, L., Star, R.A. (2003). Laser-Capture Microdissection. In: Goligorsky, M.S. (eds) Renal Disease. Methods in Molecular Medicine™, vol 86. Humana Press. https://doi.org/10.1385/1-59259-392-5:237

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  • DOI: https://doi.org/10.1385/1-59259-392-5:237

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-134-9

  • Online ISBN: 978-1-59259-392-7

  • eBook Packages: Springer Protocols

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