Renal Disease pp 237-255 | Cite as

Laser-Capture Microdissection

  • Laura Barisoni
  • Robert A. Star
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 86)

Abstract

The kidney is an anatomically complex organ with exceptional cellular heterogeneity. Our understanding of renal physiology has been advanced by studies of hand-dissected individual tubules (microdissection). Glomeruli or renal tubules are dissected away from surrounding structures, and subjected to microassays for enzymatic activity and receptor function, in vitro micro-perfusion for transport rates, and single-tubule reverse transcriptase-polymerase chain reaction (RT-PCR) (1). Thus, microdissection methods have allowed assay of activity and mRNA and protein expression in single-nephron segments in normal or uninjured kidneys. Renal disease may be global, but more typically involves selective injury to the glomerulus, portions of the nephron, interstitium, or blood vessels. Less is known about renal pathophysiology and the nephron-specific response to injury. For example, how do different portions of the nephron interact during renal injury? Why are some renal diseases focal? How do individual glomeruli react in focal semental glomerulo-sclerosis (FSGS)? Does the interaction tend to propagate or to defend against further injury? Microdissection techniques have not been widely used to study renal injury because microdissection is often limited by tissue necrosis or fibrosis. In addition, the time required for microdissection often exceeds the time-course of rapid and transient changes in a cellular response such as certain metabolic intermediates or early-response genes.

Keywords

Formalin Sucrose Hepatitis Microwave Foam 

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Copyright information

© Humana Press Inc., Totowa, NJ 2003

Authors and Affiliations

  • Laura Barisoni
    • 1
  • Robert A. Star
    • 1
  1. 1.National Institute of Diabetes and Digestive and Kidney DiseasesNational Institutes of HealthBethesda

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