Abstract
Although the best way of obtaining pure polymerase chain reaction (PCR) product will always be to optimize reaction conditions to yield only one product, there are still circumstances where DNA has to purifed from gels. Several good commercial products exist for the recovery of DNA from agarose. Here, we present a reliable method of recovering DNA from polyacrylamide gels.
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© 2003 Humana Press Inc.
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Stirling, D. (2003). Technical Notes for the Recovery and Purification of PCR Products from Acrylamide Gels. In: Bartlett, J.M.S., Stirling, D. (eds) PCR Protocols. Methods in Molecular Biology™, vol 226. Humana Press. https://doi.org/10.1385/1-59259-384-4:77
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DOI: https://doi.org/10.1385/1-59259-384-4:77
Publisher Name: Humana Press
Print ISBN: 978-0-89603-642-0
Online ISBN: 978-1-59259-384-2
eBook Packages: Springer Protocols