Skip to main content
SpringerLink
Log in

PCR Fluorescence Differential Display

  • Protocol
PCR Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 226))

  • 7066 Accesses

Abstract

Differential display of mRNA via polymerase chain reaction (DD-PCR) has become a powerful procedure for the quantitative detection of differentially expressed genes in distinct cell populations (1–4).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 74.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Liang, P., Averboukh, L., Keyomarsi, K., Sager, R., and Pardee, A. B. (1992) Differential display and cloning of messenger RNAs from human breast cancer versus mammary epithelial cells. Cancer Res. 52, 6966–6968.

    PubMed  CAS  Google Scholar 

  2. Liang, P. and Pardee, A. B. (1992) Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 257, 967–971.

    Article  PubMed  CAS  Google Scholar 

  3. Martin, K. J., Kwan, C. P., O’Hare, M. J., Pardee, A. B., and Sager, R. (1998) Identification and verification of differential display cDNAs using gene-specific primers and hybridization arrays. BioTechniques 24, 1018–1026.

    PubMed  CAS  Google Scholar 

  4. McClelland, M., Mathieu-Daude, F., and Welsh, J. (1995) RNA fingerprinting and differential display using arbitrarily primed PCR (review). Trends Genet. 11, 242–246.

    Article  PubMed  CAS  Google Scholar 

  5. Rompf, R. and Kahl, G. (1997) mRNA differential display in agarose gels. BioTechniques 23, 28–32.

    PubMed  CAS  Google Scholar 

  6. Bosch, T. C. G. and Lohmann, J. (1996) Non-radioactive differential display of messenger RNA, in Fingerprinting Methods Based on Arbitrarily Primed PCR, Springer Lab Manual (Micheli, M.R. and Bova, R., eds.), Springer Verlag, Heidelberg.

    Google Scholar 

  7. Lohmann, J., Schickle, H. P., and Bosch, T. C. G. (1995) REN, a rapid and efficient method for non-radioactive differential display and isolation of mRNA. BioTechniques 18, 200–202.

    PubMed  CAS  Google Scholar 

  8. An, G., Luo, G., Veltri, R. W., and O’Hara, S. M. (1996) Sensitive, nonradioactive differential display method using chemiluminescent detection. BioTechniques 20, 342–346.

    PubMed  CAS  Google Scholar 

  9. Ross, R., Ross, X.-I., Rueger, B., Laengin, T., and Reske-Kunz, A. B. (1999) Nonradioactive detection of differentially expressed genes using complex RNA or DNA hybridization probes. BioTechniques 26, 150–155.

    PubMed  CAS  Google Scholar 

  10. Motlik, J., Carnwath, J. W., Hermann, D., Terletski, V., Anger, M., and Niemann, H. (1998) Automated recording of RNA differential display patterns from pig granulosa cells. BioTechniques 24, 148–153.

    PubMed  CAS  Google Scholar 

  11. Bauer, D., Müller, H., Reich, J., Riedel, H., Ahrenkiel, V., Warthoe, P., et al. (1993) Identification of differentially expressed mRNA species by an improved display technique (DDRT-PCR). Nucleic Acid Res. 21, 4272–4280.

    Article  PubMed  CAS  Google Scholar 

  12. Jones, S. W. (1997) Generation of multiple mRNA fingerprints using fuorescence-based differential display and an automated DNA sequencer. BioTechniques 22, 536–543.

    PubMed  CAS  Google Scholar 

  13. Luehrsen, K. R. (1997) Analysis of differential display RT-PCR products using fluorescent primers and Genescan™ software. BioTechniques 22, 168–175.

    PubMed  CAS  Google Scholar 

  14. Reinhardt, B., Frank, U., Gellner, K., Lohmann, J., and Bosch, T. C. G. (1999) Highresolution, fluorescence-based differential display on a DNA sequencer followed by band excision. BioTechniques 27, 268–271.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Zoological Institute, University of Kiel, Kiel, Germany

    Kostya Khalturin, Sergej Kuznetsov & Thomas C. G. Bosch

Authors
  1. Kostya Khalturin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sergej Kuznetsov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Thomas C. G. Bosch
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Surgery, Glasgow Royal Infirmary, Glasgow, UK

    John M. S. Bartlett

  2. Department of Hematology, Royal Infirmary of Edinburgh, Edinburgh, UK

    David Stirling

Rights and permissions

Reprints and permissions

Copyright information

© 2003 Humana Press Inc.

About this protocol

Cite this protocol

Khalturin, K., Kuznetsov, S., Bosch, T.C.G. (2003). PCR Fluorescence Differential Display. In: Bartlett, J.M.S., Stirling, D. (eds) PCR Protocols. Methods in Molecular Biology™, vol 226. Humana Press. https://doi.org/10.1385/1-59259-384-4:237

Download citation

  • DOI: https://doi.org/10.1385/1-59259-384-4:237

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-642-0

  • Online ISBN: 978-1-59259-384-2

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation