Abstract
Since the completion of the human genome-sequencing project, scientists are now able to read the code of all human genes stored on the 46 chromosomes of the human genetic library. However, we are far from reaching an understanding of the functional relationships existing between more than a tiny fraction of these genes. The value of the human genome-sequencing project, beyond the simple collation of data, has been to teach us that it is possible to take a highly intensive analytical approach to the study of human systems in health and disease (1–4).
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References
Zhang, L., Zhou, W., Velculescu, V. E., Kern, S. E., Hruban, R. H., Hamilton, S. R., et al. (1997) Gene expression profiles in normal and cancer cells. Science 276, 1268–1272.
Lennon, G. G. (2000) High-throughput gene expression analysis for drug discovery. Drug Discovery Today 5, 59–66.
Loging, W., Lal, A., Siu, I.-M., Loney, T., Wikstrand, C., Marra, M., et al. (2000) Identifying potential tumour markers and antigens by database mining and rapid expression screening. Genome Res. 10, 1393–1402.
Szallasi, Z. (1998) Gene expression patterns and cancer. Nat. Biotechnol. 16, 1292–1293.
Larsson, M., Stahl, S., Uhlen, M., and Wennborg, A. (2000) Expression profile viewer (ExProView): A software tool for transcriptome analysis. Genomics 63, 341–353.
Lash, A. E., Tolstoshev, C. M., Wagner, L., Schuler, G. D., Strausberg, R. L., Riggins, G. J., et al. (2000) SAGEmap: A public gene expression resource. Genome Res. 10, 1051–1060.
Ji, H. J., Zhang, Q. Q., and Leung, B. S. (1990) Survey of oncogene and growth factor/receptor gene expression in cancer cells by intron-differential RNA/PCR. Biochem. Biophys. Res. Commun. 170, 569–575
Jindal, S. K., Ishii, E., Letarte, M., Vera, S., Teerds, K. J., and Dorrington, J. H. (1995) Regulation of transforming growth factor alpha gene expression in an ovarian surface epithelial cell line derived from a human carcinoma. Biol. Reprod. 52, 1027–1037.
White, B. A., ed. (1984) PCR Protocols: Current Methods & Applications.Volume 15, Methods in Molecular Biology. Humana Press, Totowa, NJ.
Liang, P. and Pardee, A. B., eds. (1998) Differential Display Methods & Protocols.Volume 85, Methods in Molecular Biology. Humana Press, Totowa, NJ.
Jurecic, R. and Belmont, J. W. (2000) Long-distance DD-PCR and cDNA microarrays. Curr. Opin. Microbiol. 3, 316–321.
Marguiles, E. and Innis, J. (2000)eSAGE: Managing and analysing data generated with serial analysis of gene expression (SAGE). Bioinformatics 16, 650–651.
Liang, P. and Pardee, A. B. (1998) Differential display: A general protocol. Mol. Biotechnol. 10, 261–267.
Jorgensen, M., Bevort, M., Kledal, T. S., Hansen, B. V., Dalgaard, M., and Leffers, H. (1999) Differential display competitive polymerase chain reaction: An optimal tool for assaying gene expression. Electrophoresis 20, 230–240.
Matz, M. V. and Lukyanov, S. A. (1998) Different strategies of differential display: Areas of application. Nucleic Acids Res. 26, 5537–5543.
Lapointe, J. and Labrie, C. (1999) Identification and cloning of a novel androgen-responsive gene, uridine diphosphoglucose dehydrogenase, in human breast cancer cells. Endocrinology 140, 4486–4493.
Cirelli, C. and Tononi, G. (1999) Differences in brain gene expression between sleep and waking as revealed by mRNA differential display and cDNA microarray technology. J. Sleep Res. 8, 44–52. Supplement.
Aittokallio, T., Ojala, P., Nevalainen, T. J., and Nevalainen, O (2000) Analysis of similarity of electrophoretic patterns in mRNA differential display. Electrophoresis 21, 2947–2956.
Lockhart, D., Dong, H., Byrne, M., Follettie, M., Gallo, M., Chee, M., et al. (1996) Expression monitoring by hybridization to high-density oligonucleotide arrays. Nat. Biotechnol. 14, 1675–1680.
Schena, M., Shalon, D., Davis, R., and Brown, P. (1995) Quantitative monitoring of gene expression patterns with a complementary cDNA microarray. Science 270, 467–470.
Lomax, M. I., Huang, L., Cho, Y., Gong, T.-W., and Altschuler, R. A. (2000) Differential display and gene arrays to examine auditory plasticity. Hearing Res. 147, 293–302.
Sun, Y. (2000) Identification and characterization of genes responsive to apoptosis: Application of DNA chip technology and mRNA differential display. Histol. Histopathol. 15, 1271–1284.
Bartlett, J. M. S. (2001) Technology evaluation: SAGE, Genzyme molecular oncology. Curr. Opin. Mol. Ther. 3, 85–96.
Kal, A. J., Van Zonneveld, A. J., Benes, V., Van den Berg, M., Koerkamp, M. G., Albermann, K., et al. (1999) Dynamics of gene expression revealed by comparison of serial analysis of gene expression transcript profiles from yeast grown on two different carbon sources. Mol. Biol. Cell 10, 1859–1872.
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Bartlett, J.M.S. (2003). Differential Display. In: Bartlett, J.M.S., Stirling, D. (eds) PCR Protocols. Methods in Molecular Biology™, vol 226. Humana Press. https://doi.org/10.1385/1-59259-384-4:217
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DOI: https://doi.org/10.1385/1-59259-384-4:217
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