Abstract
The use of quantitative polymerase chain reaction (qPCR) to detect RNA viruses has become increasingly important as a prognostic marker and in patient management, for example, in human immunodeficiency virus (HIV) and hepatitis C virus (HCV) infection. Drug therapies can be monitored by regularly checking viral load, indicating whether the regime is sufficient, or whether alternatives should be sought. It is therefore crucial that the systems used are ultrasensitive and give accurate and reproducible results.
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McDonagh, S. (2003). Ultrasensitive Quantitative PCR to Detect RNA Viruses. In: Bartlett, J.M.S., Stirling, D. (eds) PCR Protocols. Methods in Molecular Biology™, vol 226. Humana Press. https://doi.org/10.1385/1-59259-384-4:197
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DOI: https://doi.org/10.1385/1-59259-384-4:197
Publisher Name: Humana Press
Print ISBN: 978-0-89603-642-0
Online ISBN: 978-1-59259-384-2
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