PCR Protocols pp 433-444 | Cite as

Direct and Indirect In Situ PCR

  • Klaus Hermann Wiedorn
  • Torsten Goldmann
Part of the Methods in Molecular Biology™ book series (MIMB, volume 226)


In recent years, the development of in situ technologies has made good progress. In situ hybridization (ISH) has become an important tool and has enabled the pathologist to demonstrate infectious pathogens or mRNAs in tissue sections or cytospins without destruction of morphology, thus enabling the assignment of signals to individual cells or cell compartments (1, 2, 3, 4, 5, 6, 7, 8, 9).


Dextran Sulfate Nitroblue Tetrazolium Chloride Detection Buffer Buffer Paraformaldehyde Endogenous Priming 
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Copyright information

© Humana Press Inc. 2003

Authors and Affiliations

  • Klaus Hermann Wiedorn
    • 1
  • Torsten Goldmann
    • 2
  1. 1.Institut für PathologieKatharinenhospital StuttgartStuttgartGermany
  2. 2.Clinical and Experimental PathologyResearch Center BorstelBorstelGermany

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