PCR Protocols pp 401-402 | Cite as

Technical Notes for Sequencing Difficult Templates

  • David Stirling
Part of the Methods in Molecular Biology™ book series (MIMB, volume 226)


There are a number of template types that are generally recognized as being difficult to sequence. These can include sequences with a high guanine-cytosine (G/C) content, sequences that are very rich in adenine/thymine (A/T), sequences with a marked secondary structure, or large regions of homopolymer. There is no one solution to these difficulties; however, there are a number of approaches that can be used to improve the quality of sequencing data obtained from each type of problem sequence.


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Copyright information

© Humana Press Inc. 2003

Authors and Affiliations

  • David Stirling
    • 1
  1. 1.Department of HematologyRoyal Infirmary of EdinburghEdinburghUK

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