Extraction of Nucleic Acid Templates

  • John M. S. Bartlett
Part of the Methods in Molecular Biology™ book series (MIMB, volume 226)

Abstract

The following series of short technical descriptions covers the extraction of DNA and RNA from various starting materials. We have gathered these together at the beginning of this book to provide an easy reference. The polymerase chain reaction (PCR) techniques described in the rest of this book are, in the main, worked laboratory methods given detailed examples of the procedures used by the authors in their own research. However, the aim is to provide the reader with a method that may be translated into their own research; thus, although the description of ultrasensitive PCR focuses on viral genomes and cancer, this method may of course be equally applied to DNA from other sources. Rather than leaving the reader who is interested in applying this technique to ancient DNA or DNA from bone, etc., to search through the various chapters to fnd such a technique, we have collected these together for reference here.

Keywords

Formaldehyde 

References

  1. 1.
    US Department of Commerce Molecular Biology Techniques Forum http://research.nwfsc.noaa.gov/protocols/methods/
  2. 2.
  3. 3.
  4. 4.
  5. 5.
  6. 6.
    Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, New York.Google Scholar
  7. 7.
    Bartlett, J. M. S., ed. (2000) Ovarian Cancer: Methods & Protocols, Vol. 39, Methods in Molecular Biology. Humana Press, Totowa, NJ.Google Scholar

Copyright information

© Humana Press Inc. 2003

Authors and Affiliations

  • John M. S. Bartlett
    • 1
  1. 1.Endocrine Cancer Group, Division of Cancer and Molecular Pathology, University Department of SurgeryGlasgow Royal InfirmaryGlasgowUK

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