Abstract
Selectins form a group of C-type lectins, which bind to carbohydrates in a calcium dependent manner. Three selectins, E-, L-, and P-selectins are known. All selectins bind to sialyl Lewis X (sLex). E-selectin (endothelial cell adhesion molecule-1 or ELAM-1; CD62E) is expressed in the endothelial cells in response to inflammatory cytokines such as IL-1β or TNFα. E-selectin binds to the carbohydrate structure terminated by sialyl Lewisx (sLex) and sialyl Lewisa (sLea) (1). Counter receptors recognized by E-selectin are expressed by neutrophils, monocytes, eosinophils, memory T-lymphocytes, and natural killer cells. E-selectin recruits these leukocytes to the inflammatory site. Characterization of E-selectin-deficient mice confirmed a critical role of E-selectin in acute inflammatory models (2).
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© 2003 Humana Press Inc., Totowa, NJ
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Zhang, J., Fukuda, M.N. (2003). Binding Assay for Selectins. In: Buolamwini, J.K., Adjei, A.A. (eds) Novel Anticancer Drug Protocols. Methods in Molecular Medicine, vol 85. Humana Press. https://doi.org/10.1385/1-59259-380-1:117
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DOI: https://doi.org/10.1385/1-59259-380-1:117
Publisher Name: Humana Press
Print ISBN: 978-0-89603-963-6
Online ISBN: 978-1-59259-380-4
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