Abstract
Working with single-mouse embryos at the blastocyst stage is difficult from a biochemical standpoint because of the paucity of tissue. Each blastocyst contains only 25 ng of total protein (1) and has a volume of approx 160 pL (2,3). In order to measure glucose uptake, many groups have pooled embryos to increase the signal of radioactive 2-deoxyglucose transported into the blastocyst. The advantage of this technique is that single-embryo uptake can be measured using not radioactivity but enzymatic cycling reactions that are based on the amplification of a fluorescent signal. This signal is a pyridine nucleotide.
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© 2003 Humana Press Inc., Totowa, NJ
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Chi, M.MY., Moley, K.H. (2003). Single Embryo Measurement of Basal-and Insulin-Stimulated Glucose Uptake. In: Özcan, S. (eds) Diabetes Mellitus. Methods in Molecular Biology™, vol 83. Humana Press. https://doi.org/10.1385/1-59259-377-1:171
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DOI: https://doi.org/10.1385/1-59259-377-1:171
Publisher Name: Humana Press
Print ISBN: 978-1-58829-148-6
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