Abstract
The proper functioning of the insulin-producing β-cells in the pancreas depends on the action of various proteins whose function and level of expression are tightly regulated in order to maintain a well state. Alterations in these processes can lead to diseased states such as diabetes mellitus. In order to understand the complexity of these processes, it is imperative that scientists can alter the levels of expression of various genes in order to define their role in maintaining β-cell function. This can be accomplished through multiple techniques, of which the adenoviral gene transfer is the most efficient one. Gene-transfer techniques utilizing recombinant adenoviruses have become an important tool in controlling the expression level of genes of interest (1). Adenoviral gene transfer is also important in gene therapy of many inherited disorders (2). Recombinant adenoviruses are commonly used for the overexpression of transgenes and can also be used to downregulate genes of interest through the expression of antisense ribozymes and Cre-recombinase (1,2).
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Russell, W. C. (2000) Update on adenovirus and its vectors. J. Gen. Virol. 81, 2573–2604.
Kochanek, S. (1999) High-capacity adenoviral vectors for gene transfer and somatic gene therapy. Hum. Gene Then 10, 2451–2459.
Zhang, W. W. (1999) Development and application of adenoviral vectors for gene therapy of cancer. Cancer Gene Ther. 6, 113–138.
Tashiro, F, Niwa, H., and Miyazaki, J. (1999) Constructing adenoviral vectors by using the circular form of the adenoviral gene cloned in a cosmid and the Cre-loxP recombination system. Hum.. Gene Ther. 10, 1845–1852.
Bilbao, G., Contreras, J. L., Zhang, H. G., Pike, M. J., Overturf, K., Mikheeva, G., et al. (1999) Adenovirus-mediated gene expression in vivo is enhanced by the antiapoptotic bcl-2 gene. J. Virol. 73, 6992–7000.
Antinozzi, P. A., Berman, H. K., O’Doherty, R. M., and Newgard C. B. (1999) Metabolic engineering with recombinant adenoviruses. Annu. Rev. Nutr. 19, 511–544.
de Moissac, D., Zheng, H., and Kirshenbaum, L. A. (1991) Linkage of the BH4 domain of Bcl-2 and the nuclear factor kappaB signaling pathway for supression of apoptosis. J. Biol. Chem. 274, 29,505–29,509.
He, T. C, Zhou, S., da Costa, L. T, Yu, J., Kinzler, K. W., and Vogelstein, B. (1998) A simplified system for generating recombinant adenoviruses. Proc. Natl. Acad. Sci. USA 95, 2509–2514.
Kaneto, H., Xu, G., Song, K. H., Suzuma, K., Bonner-Weir, S., Sharma, A., et al. (2001) Activation of the hexosamine pathway leads to deterioration of pancreatic beta-cell function through the induction of oxidative stress. J. Biol. Chem. 276, 31,099–31,104.
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© 2003 Humana Press Inc., Totowa, NJ
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Mosley, A.L., Özcan, S. (2003). Adenoviral Gene Transfer into β-Cell Lines. In: Özcan, S. (eds) Diabetes Mellitus. Methods in Molecular Biology™, vol 83. Humana Press. https://doi.org/10.1385/1-59259-377-1:073
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DOI: https://doi.org/10.1385/1-59259-377-1:073
Publisher Name: Humana Press
Print ISBN: 978-1-58829-148-6
Online ISBN: 978-1-59259-377-4
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