Single Embryo Measurement of Basal-and Insulin-Stimulated Glucose Uptake

  • Maggie M-Y. Chi
  • Kelle H. Moley
Part of the Methods in Molecular Biology™ book series (MIMM, volume 83)


Working with single-mouse embryos at the blastocyst stage is difficult from a biochemical standpoint because of the paucity of tissue. Each blastocyst contains only 25 ng of total protein (1) and has a volume of approx 160 pL (2,3). In order to measure glucose uptake, many groups have pooled embryos to increase the signal of radioactive 2-deoxyglucose transported into the blastocyst. The advantage of this technique is that single-embryo uptake can be measured using not radioactivity but enzymatic cycling reactions that are based on the amplification of a fluorescent signal. This signal is a pyridine nucleotide.


Preimplantation Embryo Pyridine Nucleotide Leuconostoc Mesenteroides Pregnant Mare Serum Gonadotropin Human Tubal Fluid 
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Copyright information

© Humana Press Inc., Totowa, NJ 2003

Authors and Affiliations

  • Maggie M-Y. Chi
    • 1
  • Kelle H. Moley
    • 1
  1. 1.Department of Obstetrics and Gynecology, Department of Cell Biology and PhysiologyWashington University School of MedicineSt Louis

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