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Analysis of Insulin-Stimulated Glucose Uptake in Differentiated 3T3-L1 Adipocytes

  • Jaganathan Lakshmanan
  • Jeffrey S. Elmendorf
  • Sabire özcan
Part of the Methods in Molecular Biology™ book series (MIMM, volume 83)

Abstract

The long-term complications associated with diabetes such as heart disease, kidney failure, blindness, and limb amputations are mostly the result of chronic elevations in blood glucose levels, also known as chronic hyperglycemia (1,2). The exact molecular mechanisms by which hyperglycemia causes damage to various tissues are unknown. However, it is known that chronic hyperglycemia results in acute metabolic abnormalities such as insulin resistance that leads to worsening of diabetes (2). Insulin resistance results in decreased insulin-stimulated glucose transport into skeletal muscle and adipocyte tissue (3).

Keywords

Glucose Uptake Chronic Hyperglycemia Newborn Calf Serum Basal Glucose Uptake Mature White Adipocyte 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2003

Authors and Affiliations

  • Jaganathan Lakshmanan
    • 1
  • Jeffrey S. Elmendorf
    • 2
  • Sabire özcan
    • 1
  1. 1.Department of Molecular and Cellular BiochemistryUniversity of Kentucky, Medical CollegeLexington
  2. 2.Department of Cellular and Integrative PhysiologyIndiana University School of Medicine, Center for Diabetes ResearchIndianapolis

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