Analysis of Insulin-Stimulated Glucose Uptake in Differentiated 3T3-L1 Adipocytes

  • Jaganathan Lakshmanan
  • Jeffrey S. Elmendorf
  • Sabire özcan
Part of the Methods in Molecular Biology™ book series (MIMM, volume 83)


The long-term complications associated with diabetes such as heart disease, kidney failure, blindness, and limb amputations are mostly the result of chronic elevations in blood glucose levels, also known as chronic hyperglycemia (1,2). The exact molecular mechanisms by which hyperglycemia causes damage to various tissues are unknown. However, it is known that chronic hyperglycemia results in acute metabolic abnormalities such as insulin resistance that leads to worsening of diabetes (2). Insulin resistance results in decreased insulin-stimulated glucose transport into skeletal muscle and adipocyte tissue (3).


Glucose Uptake Chronic Hyperglycemia Newborn Calf Serum Basal Glucose Uptake Mature White Adipocyte 
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Copyright information

© Humana Press Inc., Totowa, NJ 2003

Authors and Affiliations

  • Jaganathan Lakshmanan
    • 1
  • Jeffrey S. Elmendorf
    • 2
  • Sabire özcan
    • 1
  1. 1.Department of Molecular and Cellular BiochemistryUniversity of Kentucky, Medical CollegeLexington
  2. 2.Department of Cellular and Integrative PhysiologyIndiana University School of Medicine, Center for Diabetes ResearchIndianapolis

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