Abstract
Well-established techniques are now in place to culture several of the major types of cells in the prostate. Epithelial cells with characteristics of basal and/or secretory luminal cells can be grown in vitro, as can stromal cells with properties of fibroblasts and/or smooth muscle. In most cases, serum-free media are available for epithelial or stromal cell cultures. Methods have been developed to establish and investigate the properties of cocultures of prostatic epithelial and stromal cells. Use of these in vitro cell cultures has provided much of the information that guides current ideas about prevention and treatment of prostate cancer.
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References
Peehl, D. M., Wong, S. T., Terris, M. K., and Stamey, T. A. (1991) Culture of prostatic epithelial cells from ultrasound-guided needle biopsies. Prostate 19, 141ā147.
Schmid, H. P. and McNeal, J. E. (1992) An abbreviated standard procedure for accurate tumor volume estimation in prostate cancer. Am. J. Surg. Pathol. 16, 184ā191.
Brothman, A. R., Patel, A. M., Peehl, D. M., and Schellhammer, P. F. (1992) Analysis of prostatic tumor cultures using fluorescence in-situ hybridization (FISH). Cancer Genet. Cytogenet. 62, 180ā185.
Chopra, D. P., Grignon, D. J., Joiakim, A., Mathieu, P. A., Mohamed, A., Sakr, W. A., et al. (1996) Differential growth factor responses of epithelial cell cultures derived from normal human prostate, benign prostatic hyperplasia, and primary prostate carcinoma. J. Cell Physiol. 169, 269ā280.
Liu, A. Y., True, L. D., LaTray, L., Ellis, W. J., Vessella, R. L., Lange, P. H., et al. (1999) Analysis and sorting of prostate cancer cell types by flow cytometry. Prostate 40, 192ā199.
Hsu, J.-Y., Feldman, D., McNeal, J. E., and Peehl, D. M. (2001) Reduced 1a-hydroxylase activity in human prostate cancer cells correlates with decreased susceptibility to 25-hydroxyvitamin D3-induced growth inhibition. Cancer Res. 61, 2852ā2856.
Brawer, M. K., Bostwick, D. G., Peehl, D. M., and Stamey, T. A. (1986) Keratin protein in human prostatic tissue and cell culture. Ann. NY Acad. Sci. 455, 729ā731.
Cussenot, O., Berthon, P., Cochand-Priollet, B., Maitland, N. J., and Le Duc, A. (1994) Immunocytochemical comparison of cultured normal epithelial prostatic cells with prostatic tissue sections. Exp. Cell Res. 214, 83ā92.
Fry, P. M., Hudson, D. L., OāHare, M. J., and Masters, J. R. (2000) Comparison of marker protein expression in benign prostatic hyperplasia in vivo and in vitro. BJU Int. 85, 504ā513.
Peehl, D. M., Wong, S. T., and Stamey, T. A. (1993) Vitamin A regulates proliferation and differentiation of human prostatic epithelial cells. Prostate 23, 69ā78.
Peehl, D. M., Leung, G. K., and Wong, S. T. (1994) Keratin expression: A measure of phenotypic modulation of human prostatic epithelial cells by growth inhibitory factors. Cell Tissue Res. 277, 11ā18.
Bayne, C. W., Donnelly, F., Chapman, K., Bollina, P., Buck, C., and Habib, F. (1998) A novel coculture model for benign prostatic hyperplasia expressing both isoforms of 5 alpha-reductase. J. Clin. Endocrinol. Metab. 83, 206ā213.
Sandhu, C., Peehl, D. M., and Slingerland, J. (2000) p16INK4A mediates cyclin dependent kinase 4 and 6 inhibition in senescent prostatic epithelial cells. Cancer Res. 60, 2616ā2622.
Wallen, E., Sellers, R. G., and Peehl, D. M. (2000) Brefeldin A induces p53-independent apoptosis in primary cultures of human prostatic cancer cells. J. Urol. 164, 836ā841.
Peehl, D. M., Erickson, E., Malspeis, L., Mayo, J., Camalier, R. F., Monks, A., et al. (1994) In vitro prostate cancer drug screen, in Fundamental Approaches to the Diagnosis and Treatment of Prostate Cancer and BPH (Imai, K., Shimazaki, J., and Karr, J. P., eds.), Academic Press, New York, pp. 57ā71.
Peehl, D. M. and Sellers, R. G. (1997) Induction of smooth muscle cell phenotype in cultured human prostatic stromal cells. Exp. Cell Res. 232, 208ā215.
Peehl, D. M. and Sellers, R. G. (1998) Basic FGF, EGF, and PDGF modify TGF-beta-induction of smooth muscle cell phenotype in human prostatic stromal cells. Prostate 35, 125ā134.
Boesch, S. T., Corvin, S., Zhang, J., Rogatsch, H., Bartsch, G., and Klocker, H. (1999) Modulation of the differentiation status of cultured prostatic smooth muscle cells by an alpha1-adrenergic receptor antagonist. Prostate 39, 226ā233.
Smith, P., Rhodes, N. P., Ke, Y., and Foster, C. S. (1999) Influence of the alpha1-adrenergic antagonist, doxazosin, on noradrenaline-induced modulation of cytoskeletal proteins in cultured hyperplastic prostatic stromal cells. Prostate 38, 216ā227.
Zhang, J., Hess, M. W., Thurnher, M., Hobisch, A., Radmayr, C., Cronauer, M. V., et al. (1997) Human prostatic smooth muscle cells in culture: estradiol enhances expression of smooth muscle cell-specific markers. Prostate 30, 117ā129.
Kassen, A., Sutkowski, D. M., Ahn, H., Sensibar, J. A., Kozlowski, J. M., and Lee, C. (1996) Stromal cells of the human prostate: initial isolation and characterization. Prostate 28, 89ā97.
Peehl, D. M., Sellers, R. G., and Wong, S. T. (1998) Defined medium for normal adult human prostatic stromal cells. In Vitro Cell Dev. Biol. Anim. 34, 555ā560.
Peehl, D. M. and Sellers, R. G. (2000) Cultured stromal cells: an in vitro model of prostatic mesenchymal biology. Prostate 45, 115ā123.
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Peehl, D.M. (2003). Growth of Prostatic Epithelial and Stromal Cells In Vitro. In: Russell, P.J., Jackson, P., Kingsley, E.A. (eds) Prostate Cancer Methods and Protocols. Methods in Molecular Medicineā¢, vol 81. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-372-0:41
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DOI: https://doi.org/10.1385/1-59259-372-0:41
Publisher Name: Springer, Totowa, NJ
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