Skip to main content

Growth of Prostatic Epithelial and Stromal Cells In Vitro

  • Protocol
Prostate Cancer Methods and Protocols

Part of the book series: Methods in Molecular Medicineā„¢ ((MIMM,volume 81))

Abstract

Well-established techniques are now in place to culture several of the major types of cells in the prostate. Epithelial cells with characteristics of basal and/or secretory luminal cells can be grown in vitro, as can stromal cells with properties of fibroblasts and/or smooth muscle. In most cases, serum-free media are available for epithelial or stromal cell cultures. Methods have been developed to establish and investigate the properties of cocultures of prostatic epithelial and stromal cells. Use of these in vitro cell cultures has provided much of the information that guides current ideas about prevention and treatment of prostate cancer.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Peehl, D. M., Wong, S. T., Terris, M. K., and Stamey, T. A. (1991) Culture of prostatic epithelial cells from ultrasound-guided needle biopsies. Prostate 19, 141ā€“147.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  2. Schmid, H. P. and McNeal, J. E. (1992) An abbreviated standard procedure for accurate tumor volume estimation in prostate cancer. Am. J. Surg. Pathol. 16, 184ā€“191.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  3. Brothman, A. R., Patel, A. M., Peehl, D. M., and Schellhammer, P. F. (1992) Analysis of prostatic tumor cultures using fluorescence in-situ hybridization (FISH). Cancer Genet. Cytogenet. 62, 180ā€“185.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  4. Chopra, D. P., Grignon, D. J., Joiakim, A., Mathieu, P. A., Mohamed, A., Sakr, W. A., et al. (1996) Differential growth factor responses of epithelial cell cultures derived from normal human prostate, benign prostatic hyperplasia, and primary prostate carcinoma. J. Cell Physiol. 169, 269ā€“280.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  5. Liu, A. Y., True, L. D., LaTray, L., Ellis, W. J., Vessella, R. L., Lange, P. H., et al. (1999) Analysis and sorting of prostate cancer cell types by flow cytometry. Prostate 40, 192ā€“199.

    ArticleĀ  PubMedĀ  Google ScholarĀ 

  6. Hsu, J.-Y., Feldman, D., McNeal, J. E., and Peehl, D. M. (2001) Reduced 1a-hydroxylase activity in human prostate cancer cells correlates with decreased susceptibility to 25-hydroxyvitamin D3-induced growth inhibition. Cancer Res. 61, 2852ā€“2856.

    PubMedĀ  CASĀ  Google ScholarĀ 

  7. Brawer, M. K., Bostwick, D. G., Peehl, D. M., and Stamey, T. A. (1986) Keratin protein in human prostatic tissue and cell culture. Ann. NY Acad. Sci. 455, 729ā€“731.

    ArticleĀ  Google ScholarĀ 

  8. Cussenot, O., Berthon, P., Cochand-Priollet, B., Maitland, N. J., and Le Duc, A. (1994) Immunocytochemical comparison of cultured normal epithelial prostatic cells with prostatic tissue sections. Exp. Cell Res. 214, 83ā€“92.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  9. Fry, P. M., Hudson, D. L., Oā€™Hare, M. J., and Masters, J. R. (2000) Comparison of marker protein expression in benign prostatic hyperplasia in vivo and in vitro. BJU Int. 85, 504ā€“513.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  10. Peehl, D. M., Wong, S. T., and Stamey, T. A. (1993) Vitamin A regulates proliferation and differentiation of human prostatic epithelial cells. Prostate 23, 69ā€“78.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  11. Peehl, D. M., Leung, G. K., and Wong, S. T. (1994) Keratin expression: A measure of phenotypic modulation of human prostatic epithelial cells by growth inhibitory factors. Cell Tissue Res. 277, 11ā€“18.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  12. Bayne, C. W., Donnelly, F., Chapman, K., Bollina, P., Buck, C., and Habib, F. (1998) A novel coculture model for benign prostatic hyperplasia expressing both isoforms of 5 alpha-reductase. J. Clin. Endocrinol. Metab. 83, 206ā€“213.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  13. Sandhu, C., Peehl, D. M., and Slingerland, J. (2000) p16INK4A mediates cyclin dependent kinase 4 and 6 inhibition in senescent prostatic epithelial cells. Cancer Res. 60, 2616ā€“2622.

    PubMedĀ  CASĀ  Google ScholarĀ 

  14. Wallen, E., Sellers, R. G., and Peehl, D. M. (2000) Brefeldin A induces p53-independent apoptosis in primary cultures of human prostatic cancer cells. J. Urol. 164, 836ā€“841.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  15. Peehl, D. M., Erickson, E., Malspeis, L., Mayo, J., Camalier, R. F., Monks, A., et al. (1994) In vitro prostate cancer drug screen, in Fundamental Approaches to the Diagnosis and Treatment of Prostate Cancer and BPH (Imai, K., Shimazaki, J., and Karr, J. P., eds.), Academic Press, New York, pp. 57ā€“71.

    Google ScholarĀ 

  16. Peehl, D. M. and Sellers, R. G. (1997) Induction of smooth muscle cell phenotype in cultured human prostatic stromal cells. Exp. Cell Res. 232, 208ā€“215.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  17. Peehl, D. M. and Sellers, R. G. (1998) Basic FGF, EGF, and PDGF modify TGF-beta-induction of smooth muscle cell phenotype in human prostatic stromal cells. Prostate 35, 125ā€“134.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  18. Boesch, S. T., Corvin, S., Zhang, J., Rogatsch, H., Bartsch, G., and Klocker, H. (1999) Modulation of the differentiation status of cultured prostatic smooth muscle cells by an alpha1-adrenergic receptor antagonist. Prostate 39, 226ā€“233.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  19. Smith, P., Rhodes, N. P., Ke, Y., and Foster, C. S. (1999) Influence of the alpha1-adrenergic antagonist, doxazosin, on noradrenaline-induced modulation of cytoskeletal proteins in cultured hyperplastic prostatic stromal cells. Prostate 38, 216ā€“227.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  20. Zhang, J., Hess, M. W., Thurnher, M., Hobisch, A., Radmayr, C., Cronauer, M. V., et al. (1997) Human prostatic smooth muscle cells in culture: estradiol enhances expression of smooth muscle cell-specific markers. Prostate 30, 117ā€“129.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  21. Kassen, A., Sutkowski, D. M., Ahn, H., Sensibar, J. A., Kozlowski, J. M., and Lee, C. (1996) Stromal cells of the human prostate: initial isolation and characterization. Prostate 28, 89ā€“97.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  22. Peehl, D. M., Sellers, R. G., and Wong, S. T. (1998) Defined medium for normal adult human prostatic stromal cells. In Vitro Cell Dev. Biol. Anim. 34, 555ā€“560.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  23. Peehl, D. M. and Sellers, R. G. (2000) Cultured stromal cells: an in vitro model of prostatic mesenchymal biology. Prostate 45, 115ā€“123.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

Ā© 2003 Humana Press Inc.

About this protocol

Cite this protocol

Peehl, D.M. (2003). Growth of Prostatic Epithelial and Stromal Cells In Vitro. In: Russell, P.J., Jackson, P., Kingsley, E.A. (eds) Prostate Cancer Methods and Protocols. Methods in Molecular Medicineā„¢, vol 81. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-372-0:41

Download citation

  • DOI: https://doi.org/10.1385/1-59259-372-0:41

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-978-0

  • Online ISBN: 978-1-59259-372-9

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics