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Studies of Local Bone Remodeling

The Calvarial Injection Assay

  • Protocol
Bone Research Protocols

Part of the book series: Methods in Molecular Medicine ((MIMM,volume 80))

Abstract

There are several assays available to study the effects of cytokines, drugs, and hormones on bone cells in vitro. However, as the complex interactions between cells are disrupted, these in vitro assays do not always reflect what happens in vivo. The calvarial injection method, originally described by Boyce et al. (1), is valuable for studying the effects of substances on bone metabolism in vivo. In this assay, the substance to be tested is injected subcutaneously over the calvarium of a mouse. At the end of the assay the animal is euthanized, and the calvarium dissected and analyzed by microscopy. Although the assay was originally used to study effects of cytokines on osteoclast formation and activity (1,2), it has also been used to study the effects of drugs on bone formation (3).

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References

  1. Boyce, B. F., Aufdemorte, T. B., Garrett, I. R., Yates, A. J., and Mundy, G. R. (1989) Effects of interleukin-1 on bone turnover in normal mice. Endocrinology 125, 1142–1150.

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  2. van’t Hof, R. J., Armour, K. J., Smith, L. M., et al. (2000) Requirement of the inducible nitric oxide synthase pathway for IL-1-induced osteoclastic bone resorption. Proc. Natl. Acad. Sci. USA 97, 7993–7998.

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  3. Mundy, G., Garrett, R., Harris, S., et al. (1999) Stimulation of bone formation in vitro and in rodents by statins. Science 286, 1946–1949.

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Author information

Authors and Affiliations

  1. Bone Research Group, Department of Medicine and Therapeutics, University of Aberdeen Medical School, Aberdeen, UK

    Robert J. van’t Hof, Claire E. Clarkin & Kenneth J. Armour

Authors
  1. Robert J. van’t Hof
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  2. Claire E. Clarkin
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  3. Kenneth J. Armour
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Editor information

Editors and Affiliations

  1. University of Aberdeen Medical School, Aberdeen, UK

    Miep H. Helfrich PhD  & Stuart H. Ralston MD  & 

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© 2003 Humana Press Inc., Totowa, NJ

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van’t Hof, R.J., Clarkin, C.E., Armour, K.J. (2003). Studies of Local Bone Remodeling. In: Helfrich, M.H., Ralston, S.H. (eds) Bone Research Protocols. Methods in Molecular Medicine, vol 80. Humana Press. https://doi.org/10.1385/1-59259-366-6:345

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  • DOI: https://doi.org/10.1385/1-59259-366-6:345

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-044-1

  • Online ISBN: 978-1-59259-366-8

  • eBook Packages: Springer Protocols

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