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Transmission Electron Microscopy of Bone

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Bone Research Protocols

Part of the book series: Methods in Molecular Medicine ((MIMM,volume 80))

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Abstract

Ultrastructural analysis of bone and other mineralized tissues such as calcified cartilage and dentin is essential for the understanding of the cell-cell/cell-matrix interaction, composition, and three-dimensional organization of these tissues. A wide variety of techniques have been introduced to process such tissues. This chapter describes a few methods to process mineralized tissues obtained from different sources. In addition, attention is paid to processing of cultured bone explants for electron microscopic analysis.

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References

  1. Gruber, H. E. (1992) Adaptations of Goldner’s Masson trichrome stain for the study of undecalcified plastic embedded bone. Biotech. Histochem. 67, 30–34.

    Article  PubMed  CAS  Google Scholar 

  2. Rungby, J., Kassem, M., Eriksen, E. F., and Danscher, G. (1993) The von Kossa reaction for calcium deposits: silver lactate staining increases sensitivity and reduces background. Histochem. J. 25, 446–451.

    Article  PubMed  CAS  Google Scholar 

  3. Reynolds, E. S. (1963) The use of lead nitrate at high pH as an electron opaque stain in electron microscopy. J. Cell Biol. 17, 208.

    Article  PubMed  CAS  Google Scholar 

  4. Everts, V., Korper, W., Jansen, D. C., et al. (1999) Functional heterogeneity of osteoclasts: matrix metalloproteinases participate in osteoclastic resorption of calvarial bone but not in resorption of long bone. FASEB J. 13, 1219–1230.

    PubMed  CAS  Google Scholar 

  5. Landis, W. J., Paine, M. C., and Glimcher, M. J. (1978) Use of acrolein vapors for anhydrous preparation of bone tissue for electron microscopy. J. Ultrastruct. Res. 70, 171–180.

    Article  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Cell Biology and Histology, Academic Medical Centre, The University of Amsterdam, Amsterdam, The Netherlands

    Vincent Everts

  2. Department of Periodontology, Academic Centre for Dentistry Amsterdam, The University of Amsterdam, Amsterdam, The Netherlands

    Vincent Everts, Anneke Niehof & Wouter Beertsen

Authors
  1. Vincent Everts
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  2. Anneke Niehof
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  3. Wouter Beertsen
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Editor information

Editors and Affiliations

  1. University of Aberdeen Medical School, Aberdeen, UK

    Miep H. Helfrich PhD  & Stuart H. Ralston MD  & 

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© 2003 Humana Press Inc., Totowa, NJ

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Everts, V., Niehof, A., Beertsen, W. (2003). Transmission Electron Microscopy of Bone. In: Helfrich, M.H., Ralston, S.H. (eds) Bone Research Protocols. Methods in Molecular Medicine, vol 80. Humana Press. https://doi.org/10.1385/1-59259-366-6:299

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  • DOI: https://doi.org/10.1385/1-59259-366-6:299

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-044-1

  • Online ISBN: 978-1-59259-366-8

  • eBook Packages: Springer Protocols

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