Abstract
This chapter describes the practical aspects of performing cytogenetic studies in a variety of lymphoid disorders, including the lymphomas, multiple myeloma, chronic lymphocytic leukemia, and other chronic lymphoproliferative diseases. They are also required for studies of acute lymphoblastic leukemia of mature T-cell or B-cell types. As mentioned in the previous chapter, most lymphoid cells are either T-lineage or B-lineage. During normal differentiation, they become capable of responding to antigens, and one of these responses is to transform and undergo division. A variety of reagents (known as mitogens) with antigenic properties are used in the laboratory to stimulate the cells to transform in a similar way. The two mitogens featured in the methods described here are Phytohemagglutinin (PHA) for T-cells (see Note 1), and 12-O-tetradecanoyl-phorbol-13-acetate (TPA) for B cells. Other B-cell mitogens are described in Note 2. Be aware that no mitogen is absolutely specific to T cells or B cells (see Note 3).
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© 2003 Humana Press Inc.
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Swansbury, J. (2003). Other Lymphoid Malignancies. In: Swansbury, J. (eds) Cancer Cytogenetics. Methods in Molecular Biology™, vol 220. Humana Press. https://doi.org/10.1385/1-59259-363-1:111
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DOI: https://doi.org/10.1385/1-59259-363-1:111
Publisher Name: Humana Press
Print ISBN: 978-1-58829-080-9
Online ISBN: 978-1-59259-363-7
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