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Drugs of Abuse pp 405-412 | Cite as

Organotypic Culture of Developing Striatum

Pharmacological Induction of Gene Expression
  • Fu-Chin Liu
Part of the Methods In Molecular Medicine™ book series (MIMM, volume 79)

Abstract

The major advantage of the organotypic culture of striatal slices is that the organotypic culture preserves much of the physiologically relevant environment of striatal neurons. The organotypic culture represents a system more anatomically and physiologically relevant than cultures of cell lines and dissociated primary striatal cells (1,2). The maintenance of the infrastructure of cell-cell interactions in striatal slices allows analysis of neuronal development and cell biology of striatal neurons in an approximately in situ condition. This special property and the experimentally accessible condition of cells in the slices makes organotypic cultures of the striatum a value compromise paradigm between in vivo whole animal and in vitro dissociated cell culture approaches.

Keywords

Induction Time Striatal Neuron Organotypic Culture Laminar Flow Hood Striatal Slice 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Stoppini, L., Buchs, P. A., and Muller, D. (1991) A simple method for organotypic cultures of nervous tissue. J. Neurosci. Methods 37, 173–182.PubMedCrossRefGoogle Scholar
  2. 2.
    Gähwiler, B. H., Capogna, M., Debanne, D., McKinney, R. A., and Thompson, S. M. (1997) Organotypic slice cultures: a technique has come of age. Trends Neurosci. 20, 471–477.PubMedCrossRefGoogle Scholar
  3. 3.
    Graybiel, A. M. (1990) Neurotransmitters and neuromodulators in the basal ganglia. Trends Neurosci. 13, 244–254.PubMedCrossRefGoogle Scholar
  4. 4.
    Gerfen, C. R. (1992) The neostriatal mosaic: multiple levels of compartmental organization in the basal ganglia. Annu. Rev. Neurosci. 15, 285–320.PubMedCrossRefGoogle Scholar
  5. 5.
    Graybiel, A. M. and Hickey, T. L. (1982) Chemospecificity of ontogenetic units in the striatum: demonstration by combining [3H] thymidine neuronography and histochemical staining. Proc. Natl. Acad. Sci. USA 79, 198–202.PubMedCrossRefGoogle Scholar
  6. 6.
    van der Kooy, D. and Fishell, G. (1987) Neuronal birthdate underlies the development of striatal compartments. Brain Res. 401, 155–161.PubMedCrossRefGoogle Scholar
  7. 7.
    Liu, F.-C., Takahashi, H., McKay, R. D. G., and Graybiel, A. M. (1995) Dopaminergic regulation of transcription factor expression in organotypic cultures of developing striatum. J. Neurosci. 15, 2367–2384.PubMedGoogle Scholar
  8. 8.
    Liu, F.-C. and Graybiel, A. M. (1996) Spatiotemporal dynamics of CREB phosphorylation: transient versus sustained phosphorylation in the developing striatum. Neuron 17, 1133–1144.PubMedCrossRefGoogle Scholar
  9. 9.
    Liu, F.-C. and Graybiel, A. M. (1998) Region-dependent dynamics of cAMP response element-binding protein phosphorylation in the basal ganglia. Proc. Natl. Acad. Sci. USA 95, 4708–4713.PubMedCrossRefGoogle Scholar
  10. 10.
    Liu, F.-C. and Graybiel, A. M. (1998) Activity-regulated phosphorylation of cAMP response element binding protein in the developing striatum: implications for patterning the neurochemical phenotypes of striatal compartments. Dev. Neurosci. 20, 229–236.PubMedCrossRefGoogle Scholar
  11. 11.
    Segal, R. A., Takahashi, H., and McKay, R. D. G. (1992) Changes in neurotrophin responsiveness during the development of cerebellar granule neurons. Neuron 9, 1041–1052.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2003

Authors and Affiliations

  • Fu-Chin Liu
    • 1
  1. 1.Institute of NeuroscienceNational Yang-Ming UniversityTaipeiTaiwan

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