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Drugs of Abuse pp 193-209 | Cite as

Analysis of mRNA Expression in Striatal Tissue by Differential Display Polymerase Chain Reaction

  • Joshua D. Berke
Part of the Methods In Molecular Medicine™ book series (MIMM, volume 79)

Abstract

Differential display polymerase chain reaction (DDPCR) is a technique that allows comparisons between the expressed mRNA population in two or more tissues, or in the same tissue under two or more different conditions. For example, it has been used to discover striatal genes whose expression is altered following a drug injection (1,2). The essential idea behind DDPCR is to generate an RNA “fingerprint,” a pattern of bands corresponding to the expressed mRNA population. By performing parallel sets of reactions for each experimental condition and displaying the resulting fingerprints side by side, bands that differ between conditions can be noted and the corresponding mRNA identified.

Keywords

Custom Primer Upstream Primer Reverse Transcriptase Reaction Geiger Counter Differential Display Polymerase Chain Reaction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2003

Authors and Affiliations

  • Joshua D. Berke
    • 1
  1. 1.Laboratory of Cognitive Neurobiology, Department of PsychologyBoston UniversityBoston

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