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Fibroblastic, Hematopoietic, and Hormone Responsive Epithelial Cell Lines and Culture Conditions for Elucidation of Signal Transduction and Drug Resistance Pathways by Gene Transfer

  • Protocol
Cancer Cell Signaling

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 218))

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Abstract

Elucidation of signal transduction pathways involved in proliferation, cell cycle progression and the regulation of apoptosis has shown great promise in the treatment of various diseases including neoplastic, inflammatory, autoimmune, immunodeficiency, arthritic and neurodegenerative disorders. By understanding how these signal transduction pathways function, chemotherapeutic targets may be identified which will suppress or eliminate the disease. This information may eventually be translated into therapy, which would either eliminate or safely contain the patient’s disease. This chapter will focus on basic tissue culture techniques which are used to elucidate signal transduction pathways. Furthermore, this chapter will provide a general background for understanding how gene transfer techniques can be used to elucidate signal transduction pathways as well as various pitfalls commonly encountered with their usage.

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Author information

Authors and Affiliations

  1. Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, NC

    Linda S. Steelman, Phillip W. Moye, John T. Lee, John G. Shelton, Patrick M. Navolanic, Steven L. Knapp, Richard A. Franklin & James A. McCubrey

  2. Shands Cancer Center, University of Florida, Gainesville, FL

    William L. Blalock

  3. BD Biosciences Clontech, Palo Alto, CA

    Xiao-Yang Wang

  4. Department of Biology, East Carolina University, Greenville, NC

    Julianne M. Davis

  5. Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson College of Medicine, Philadelphia, PA

    Martyn K. White

Authors
  1. Linda S. Steelman
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  2. William L. Blalock
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  3. Xiao-Yang Wang
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  4. Phillip W. Moye
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  5. John T. Lee
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  6. John G. Shelton
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  7. Patrick M. Navolanic
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  8. Julianne M. Davis
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  9. Steven L. Knapp
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  10. Richard A. Franklin
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  11. Martyn K. White
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  12. James A. McCubrey
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Editor information

Editors and Affiliations

  1. Brody School of Medicine, East Carolina University, Greenville, NC

    David M. Terrian

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© 2003 Humana Press Inc., Totowa, NJ

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Steelman, L.S. et al. (2003). Fibroblastic, Hematopoietic, and Hormone Responsive Epithelial Cell Lines and Culture Conditions for Elucidation of Signal Transduction and Drug Resistance Pathways by Gene Transfer. In: Terrian, D.M. (eds) Cancer Cell Signaling. Methods in Molecular Biology™, vol 218. Humana Press. https://doi.org/10.1385/1-59259-356-9:185

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  • DOI: https://doi.org/10.1385/1-59259-356-9:185

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-075-5

  • Online ISBN: 978-1-59259-356-9

  • eBook Packages: Springer Protocols

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