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Cell Therapy in the Heart

Cell Production, Transplantation, and Applications

  • Protocol
Cardiac Cell and Gene Transfer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 219))

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Abstract

Cardiac dysfunction resulting from various insults to the myocardium can ultimately lead to the development of heart failure. Owing to the limited regenerative capacity of adult cardiac tissue and the lack of a resident cardiac progenitor cell equivalent to the skeletal myoblast, the transplantation of myogenic cells into the myocardium has been investigated as a novel mechanism to repair damaged and dysfunctional myocardium. For maximal impact on cardiac function, the design of cellular therapies must carefully consider the identity of the transplanted cells as well as the method of cell delivery. This review details techniques for the purification of commonly investigated cell grafts, including skeletal myoblasts and mesenchymal stem cells, and the methods to deliver these grafts to the myocardium of laboratory animals.

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Author information

Authors and Affiliations

  1. Departments of Pediatrics and Molecular Genetics and Microbiology, University of Florida School of Medicine, Gainesville, FL

    Kevin S. Cahill & Barry J. Byrne

  2. Division of Cardiology, Peter Belfer Laboratory, Johns Hopkins University School of Medicine, Baltimore, MD

    Catalin Toma

  3. Osiris Therapeutics Inc., Baltimore, MD

    Mark F. Pittenger

  4. Peter Belfer Laboratory, Division of Cardiology, Johns Hopkins University School of Medicine, Baltimore, MD

    Paul D. Kessler

Authors
  1. Kevin S. Cahill
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  2. Catalin Toma
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  3. Mark F. Pittenger
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  4. Paul D. Kessler
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  5. Barry J. Byrne
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Editor information

Editors and Affiliations

  1. Department of Physiology, Department of Internal Medicine, University of Michigan, Ann Arbor, MI

    Joseph M. Metzger (Director) (Director)

  2. Center for Integrative Genomics, University of Michigan, Ann Arbor, MI

    Joseph M. Metzger (Director) (Director)

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© 2003 Humana Press Inc.

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Cahill, K.S., Toma, C., Pittenger, M.F., Kessler, P.D., Byrne, B.J. (2003). Cell Therapy in the Heart. In: Metzger, J.M. (eds) Cardiac Cell and Gene Transfer. Methods in Molecular Biology, vol 219. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-350-X:73

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  • DOI: https://doi.org/10.1385/1-59259-350-X:73

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-994-0

  • Online ISBN: 978-1-59259-350-7

  • eBook Packages: Springer Protocols

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