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Dual Vector Expansion of the Recombinant AAV Packaging Capacity

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Book cover Cardiac Cell and Gene Transfer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 219))

Abstract

Adeno-associated virus (AAV) as a vector for gene therapy of cardiovascular diseases has received much recent attention (1–6). This enthusiasm is based on the success of numerous proof of principal gene transfer experiments. Stable high-level expression has been achieved following a variety of delivery methods (direct cardiac muscle injection, coronary vascular or carotid artery infection) and in a wide range of animal models from rodents to pigs (7–12). A side-by-side comparison between viral and nonviral vectors in rabbit heart suggests that rAAV is much more efficient than nonviral vectors such as naked or liposome-complexed DNA. At the same time, rAAV also displays the least inflammatory response in comparison with other viral vectors such as adenovirus and herpes simplex virus (13). Regulated myocardial transgene expression has also been demonstrated with an AAV vector containing glucocorticoid response elements in rat heart (14).

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Author information

Authors and Affiliations

  1. Department of Molecular Microbiology and Immunology School of Medicine, University of Missouri, Columbia, MO

    Dongsheng Duan & Yongping Yue

  2. Department of Anatomy and Cell Biology, University of Iowa College of Medicine, Iowa City, IA

    John F. Engelhardt

Authors
  1. Dongsheng Duan
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  2. Yongping Yue
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  3. John F. Engelhardt
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Editors and Affiliations

  1. Department of Physiology, Department of Internal Medicine, University of Michigan, Ann Arbor, MI

    Joseph M. Metzger (Director) (Director)

  2. Center for Integrative Genomics, University of Michigan, Ann Arbor, MI

    Joseph M. Metzger (Director) (Director)

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© 2003 Humana Press Inc.

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Duan, D., Yue, Y., Engelhardt, J.F. (2003). Dual Vector Expansion of the Recombinant AAV Packaging Capacity. In: Metzger, J.M. (eds) Cardiac Cell and Gene Transfer. Methods in Molecular Biology, vol 219. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-350-X:29

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  • DOI: https://doi.org/10.1385/1-59259-350-X:29

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-994-0

  • Online ISBN: 978-1-59259-350-7

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