Suppressor tRNAs

Protocols and Applications for Cardiac Gene Transfer
  • Massimo Buvoli
  • Ada Buvoli
  • Leslie A. Leinwand
Part of the Methods in Molecular Biology book series (MIMB, volume 219)


Although tRNA-mediated suppression has been mainly used to study chainterminating mutations in bacteria and yeast (1,2), suppressor tRNAs have also been employed for a variety of other purposes. For example, by introducing a nonsense mutation into the diphtheria toxin-coding sequence, its expression in vivo can be regulated by the presence or absence of a suppressor tRNA. This technique has provided new approaches to cancer therapy (3) as well as to the study of visual system development in Drosophila melanogaster (4).


Nonsense Mutation Cyanoacrylate Adhesive Cesium Chloride Polymerase Chain Reaction Solution Suppressor tRNA 
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  1. 1.
    Steege, D. A. and Soll, D. G. (1979) Suppression, in Biological Regulation and Development, (Goldberger R. F., ed.), Plenum, New York, pp. 433–475.Google Scholar
  2. 2.
    Hinnebusch, A. G. and Liebman, S. W. (1991) Protein synthesis and translational control in Saccharomyces cerevisiae, in The Molecular and Cellular Biology of the Yeast Saccharomyces: Genome Dynamics, Protein synthesis, and Energetics (Broach, J. R., Pringle, J. R., and Jones, E. W., eds.), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp. 672–686.Google Scholar
  3. 3.
    Robinson, D. F. and Maxwell, I. H. (1995) Suppression of single and double nonsense mutations introduced into diphtheria toxin A-chain gene: a potential binary system for toxin gene therapy. Hum. Gene Ther. 6, 137–143.PubMedCrossRefGoogle Scholar
  4. 4.
    Kunes, S. and Steller H. (1991) Ablation of Drosophila photoreceptor cells by conditional expression of a toxin gene. Genes Dev. 5, 970–983.PubMedCrossRefGoogle Scholar
  5. 5.
    Atkinson, J. and Martin, R. (1994). Mutations to nonsense codons in human genetic disease: implications for gene therapy by nonsense suppressor tRNAs. Nucleic Acids Res. 22, 1327–1334.PubMedCrossRefGoogle Scholar
  6. 6.
    Temple, G. F., Dozy, A. M., Roy, K. L., and Kan, Y. W. (1982) Construction of a functional human suppressor tRNA gene: an approach to gene therapy for β-thalassaemia. Nature 296, 537–540.PubMedCrossRefGoogle Scholar
  7. 7.
    Panchal, R. G., Wang, S., McDermott, J., and Link, C. J., Jr. (1999) Partial functional correction of xeroderma pigmentosum group A cells by suppressor tRNA. Hum. Gene Ther. 10, 2209–2219.PubMedCrossRefGoogle Scholar
  8. 8.
    Buvoli, M., Buvoli, A., and Leinwand, L.A. (2000) Suppression of nonsense mutations in cell culture and mice by multimerized suppressor tRNA genes. Mol. Cell. Biol. 20, 3116–3124.PubMedCrossRefGoogle Scholar
  9. 9.
    Hudziak, R. M., Laski, U. L., RajBhandary, U. L., Sharp P. A., and Capecchi, M. R. (1982). Establishment of mammalian cell line containing multiple nonsense mutations and functional suppressor tRNA genes. Cell 31, 137–146.PubMedCrossRefGoogle Scholar
  10. 10.
    Capone, J. P., Sharp, P. A., and RajBhandary, U. L. (1985) Amber, ochre and opal suppressor tRNA genes derived from a human serine tRNA gene. EMBO J. 4, 213–221.PubMedGoogle Scholar
  11. 11.
    Li, K., Welikson, R. E., Vikstrom, K. L., and Leinwand, L. A. (1997) Direct gene transfer into the mouse heart. J. Mol. Cell. Cardiol. 29, 1499–1504.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 2003

Authors and Affiliations

  • Massimo Buvoli
    • 1
  • Ada Buvoli
    • 1
  • Leslie A. Leinwand
    • 1
  1. 1.Department of Molecular, Cellular and Developmental BiologyUniversity of Colorado at BoulderBoulder

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