Although tRNA-mediated suppression has been mainly used to study chainterminating mutations in bacteria and yeast (1,2), suppressor tRNAs have also been employed for a variety of other purposes. For example, by introducing a nonsense mutation into the diphtheria toxin-coding sequence, its expression in vivo can be regulated by the presence or absence of a suppressor tRNA. This technique has provided new approaches to cancer therapy (3) as well as to the study of visual system development in Drosophila melanogaster (4).
KeywordsNonsense Mutation Cyanoacrylate Adhesive Cesium Chloride Polymerase Chain Reaction Solution Suppressor tRNA
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