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Suppressor tRNAs

Protocols and Applications for Cardiac Gene Transfer
  • Massimo Buvoli
  • Ada Buvoli
  • Leslie A. Leinwand
Part of the Methods in Molecular Biology book series (MIMB, volume 219)

Abstract

Although tRNA-mediated suppression has been mainly used to study chainterminating mutations in bacteria and yeast (1,2), suppressor tRNAs have also been employed for a variety of other purposes. For example, by introducing a nonsense mutation into the diphtheria toxin-coding sequence, its expression in vivo can be regulated by the presence or absence of a suppressor tRNA. This technique has provided new approaches to cancer therapy (3) as well as to the study of visual system development in Drosophila melanogaster (4).

Keywords

Nonsense Mutation Cyanoacrylate Adhesive Cesium Chloride Polymerase Chain Reaction Solution Suppressor tRNA 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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Copyright information

© Humana Press Inc. 2003

Authors and Affiliations

  • Massimo Buvoli
    • 1
  • Ada Buvoli
    • 1
  • Leslie A. Leinwand
    • 1
  1. 1.Department of Molecular, Cellular and Developmental BiologyUniversity of Colorado at BoulderBoulder

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