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Detection of Pathogenic Yersinia enterocolitica by a Swab Enrichment PCR Procedure

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PCR Detection of Microbial Pathogens

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 216))

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Abstract

The Gram-negative Yersinia genus belongs to the family of Enterobacteriaceae, in which 3 of the 11 Yersinia species are recognized as human pathogens, namely Y. pestis (the etiological factor of plague), Y. pesudotuberculosis, and Y. enterocolitica (the causative agents for yersiniosis) (1). The rest of the genus is composed of Y. ruckeri (a fish pathogen) and the Y. enterocolitica-like organisms (2,3), which have been biochemically classified as 7 species: Y. fredriksenii, Y. kristensenii, Y. intermedia, Y. aldovae, Y. rohdei, Y. mollaretii, and Y. bercoveri (4–7). The heterogeneous composition of Y. enterocolitica, with respect to phenotypic and genotypic properties, is problematic from an analytical point of view; the species being distinguished by 6 biogroups (8) and more than 60 serotypes (9).

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Author information

Authors and Affiliations

  1. Applied Microbiology, Center for Chemistry and Chemical Engineering, Lund University, Lund, Sweden

    Rickard Knutsson

  2. Danish Veterinary Institute, Copenhagen, Denmark

    Peter Rådström

Authors
  1. Rickard Knutsson
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  2. Peter Rådström
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Editor information

Editors and Affiliations

  1. Federal Institute for Health Protection of Consumers and Veterinary Medicine (BgVV), Jena, Germany

    Konrad Sachse (Dr. Rer. Nat.) (Dr. Rer. Nat.)

  2. University of Bern, Bern, Switzerland

    Joachim Frey PhD

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© 2003 Humana Press Inc., Totowa, NJ

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Knutsson, R., Rådström, P. (2003). Detection of Pathogenic Yersinia enterocolitica by a Swab Enrichment PCR Procedure. In: Sachse, K., Frey, J. (eds) PCR Detection of Microbial Pathogens. Methods in Molecular Biology™, vol 216. Humana Press. https://doi.org/10.1385/1-59259-344-5:311

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  • DOI: https://doi.org/10.1385/1-59259-344-5:311

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-049-6

  • Online ISBN: 978-1-59259-344-6

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